Sewage sludge composted and bio-promoters enhance soil nutrient availability and initial development of Astronium fraxinifolium Schott

Abstract

This study evaluated the effects of sewage sludge composted (SSC, mixed with sugarcane bagasse at 1:1 ratio) and microbial biopromoters on soil fertility and early growth of Astronium fraxinifolium, a native tree with high recovery potential in the Brazilian Cerrado. In a randomized block design (2 × 4 factorial), treatments consisted of two SSC levels (with and without 23 g pot⁻¹ of chemically characterized, environmentally safe compost) and four inoculation treatments (control; Bacillus subtilis - BS; Rhizophagus clarus - RhC; and BS + RhC) with five replicates in 6-L pots. After 150 days, growth, root morphology, gas exchange, and soil properties were evaluated. SSC improved soil fertility (reduced aluminum, increased P, sum of bases, base saturation, and essential micronutrients Fe, Mn, Zn) without inducing heavy metal toxicity, increasing plant height. The SSC + RhC interaction significantly promoted shoot and root biomass, stem diameter, and root surface area/volume. Seedling Quality Index (SQI) was highest under SSC + RhC and SSC + BS. Furthermore, SSC and inoculants significantly enhanced gas exchange parameters, translating into substantially higher photosynthetic performance. Overall, combining this specific SSC rate with biopromoters, particularly Rh. clarus, effectively improved soil chemical properties and seedling vigor. While limited to nursery conditions, these findings demonstrate that this approach successfully promotes high-quality seedlings, establishing a promising baseline for future long-term field validation and dose-response trials in degraded lands.