2026· Methods in molecular biology· Vol 3005, pp.
23-56
· 0 citations
Medicine
TL;DR
A detailed protocol for flap-enabled next-generation capture (FENGC), a cost-effective method for targeted, multiplexed enrichment of DNA sequences for epigenetic and genetic analysis.
Fiber-seq simultaneously profiles chromatin accessibility, DNA methylation, protein footprints, and genetic variation on single molecules at near—base-pair resolution, revealing how genetic and epigenetic features interact to regulate gene expression and provides a powerful new framework for dissecting immune cell func...
Emily A. Madden, James T. Anderson, M. Cowles et al.· Journal of Immunology· 0 citations
Chromatin accessibility has long been used as a marker for regions of DNA with regulatory potential. Fiber-seq detects chromatin accessibility on individual DNA fibers, enabling analyses beyond the identification of the accessible chromatin regions (ACRs). By providing single molecule level high resolution, Fiber-seq p...
K. Bubb, Molly Perchlik, J. Cuperus et al.· bioRxiv· 0 citations
In a recent study published in Nature, Chen et al. introduced CHARM (single-cell assay for Chromatin conformation, Histone modi fi cation, chromatin Accessibility, and RNA expression Multi-omics pro fi ling), a platform that simultaneously captures four regulatory modalities within the same nucleus. 1 This integrated str...
Hakjin Kim, Jongwon Byun, Taeho Kwon· Signal Transduction and Targ...· 0 citations
A comprehensive ATAC-seq protocol that is utilized by laboratories, along with detailed troubleshooting steps at each point, is outlined in the hopes that it will aid other researchers in utilizing this tool to the fullest capability.
Kaitlyn Gura, T. Long, Motoki Takaku et al.· Methods in molecular biology· 0 citations
A detailed CUT&RUN protocol from sample collection through data analysis, including best practices and defined controls to ensure specific, efficient, and robust target profiling is provided.
Tessa M. Firestone, Bryan J. Venters, Katherine Novitzky et al.· Methods in molecular biology· 0 citations
The mechanisms and roles of DNA methylation in epigenetic regulation are examined, the current landscape of DNA methylation modulators are evaluated, from traditional DNMT inhibitors to cutting-edge CRISPR-dCas9 fusion systems and protein-protein interaction disruptors, and their clinical relevance are evaluated.
Julie Gilbert, Francesco Calzaferri· Chemical Research in Toxicol...· 0 citations
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