Skip to content

FENGC: Targeted, Multiplexed, and Integrated Single-Molecule Profiling of Chromatin Accessibility and DNA Methylation.

2026 · Methods in molecular biology · Vol 3005, pp. 23-56 · 0 citations
Medicine

TL;DR

A detailed protocol for flap-enabled next-generation capture (FENGC), a cost-effective method for targeted, multiplexed enrichment of DNA sequences for epigenetic and genetic analysis.

View source

Similar papers

Open access Jul 2026

Unraveling genomic regulatory complexity by adding chromatin accessibility to the long-read sequencing toolkit 2247245

Fiber-seq simultaneously profiles chromatin accessibility, DNA methylation, protein footprints, and genetic variation on single molecules at near—base-pair resolution, revealing how genetic and epigenetic features interact to regulate gene expression and provides a powerful new framework for dissecting immune cell func...

Emily A. Madden, James T. Anderson, M. Cowles et al. · 0 citations
Open access Aug 2026

Unpacking Chromatin Accessibility with Fiber-seq

Chromatin accessibility has long been used as a marker for regions of DNA with regulatory potential. Fiber-seq detects chromatin accessibility on individual DNA fibers, enabling analyses beyond the identification of the accessible chromatin regions (ACRs). By providing single molecule level high resolution, Fiber-seq p...

K. Bubb, Molly Perchlik, J. Cuperus et al. · 0 citations
Open access Jul 2026

Decoding the regulatory genome: single-cell four-omics integration

In a recent study published in Nature, Chen et al. introduced CHARM (single-cell assay for Chromatin conformation, Histone modi fi cation, chromatin Accessibility, and RNA expression Multi-omics pro fi ling), a platform that simultaneously captures four regulatory modalities within the same nucleus. 1 This integrated str...

Hakjin Kim, Jongwon Byun, Taeho Kwon · 0 citations
2026

Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq) of Cancer Cells in Culture.

A comprehensive ATAC-seq protocol that is utilized by laboratories, along with detailed troubleshooting steps at each point, is outlined in the hopes that it will aid other researchers in utilizing this tool to the fullest capability.

Kaitlyn Gura, T. Long, Motoki Takaku et al. · 0 citations
Open access 2026

High-Efficiency Genomic Mapping of Chromatin-Associated Targets with CUT&RUN.

A detailed CUT&RUN protocol from sample collection through data analysis, including best practices and defined controls to ensure specific, efficient, and robust target profiling is provided.

Tessa M. Firestone, Bryan J. Venters, Katherine Novitzky et al. · 0 citations
Review Open access Jul 2026

Targeting DNA Methylation: New Paradigms and the Advent of Gene-Selective Tools.

The mechanisms and roles of DNA methylation in epigenetic regulation are examined, the current landscape of DNA methylation modulators are evaluated, from traditional DNMT inhibitors to cutting-edge CRISPR-dCas9 fusion systems and protein-protein interaction disruptors, and their clinical relevance are evaluated.

Julie Gilbert, Francesco Calzaferri · 0 citations

We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.