Improving expression of Photobacterium sp. JT-ISH-224 α2,6-sialyltransferase using ProteinMPNN and machine learning guided combinatory mutagenesis

Machine learning-assisted directed evolution of plant Rubisco

The utility of ML-assisted evolution for engineering Rubisco with improved carboxylation efficiency and potential for enhancing crop productivity is demonstrated.

From Computational Screening to Preparative Scale: A Transaminase Identified by Machine Learning for (R)-1-Boc-3-aminopyrrolidine

Deep learning-driven de novo design of ribosome binding sites in Paracoccus denitrificans

This work establishes the first deep learning-enabled RBS design platform for P. denitrificans, offering a robust tool for precise translational regulation and advancing synthetic biology applications in environmental biotechnology.

Engineering a highly active thermophilic F1-ATPase by homolog-guided exploration and machine-learning-assisted prioritization

The rotary motor F1-ATPase has been extensively studied as a model molecular machine, yet rational engineering of its catalytic activity remains challenging because ATP hydrolysis is regulated by long-range intersubunit allostery and large conformational transitions. Here, we developed a homolog-guided engineering stra...

Machine-Learning-Enabled Rapid Evolution of Photoenzymes for the Asymmetric Synthesis of gem-Difluorophosphonates.

A small-sample, accelerated evolution strategy that integrates focused rational iterative site-specific mutagenesis (FRISM) with the EVOLVEpro model is reported, providing a robust, "lightweight" machine learning framework for the rapid development of new-to-nature photoenzymatic transformations.

Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization

Dalbavancin is a potent next-generation lipoglycopeptide antibiotic, but the biosynthesis of its essential precursor, A40926B0, is severely hindered in its native producer Nonomuraea gerenzanensis. Complex competing metabolic pathways and strict transcriptional repression in this strain lead to critical industrial bott...