Aug 2026· Biosensors· Vol 16· 1 citation· 41 references
Medicine
Abstract
Staphylococcus aureus (S. aureus) is a common foodborne pathogen that can cause the severe contamination of dairy products. Therefore, there is an urgent need for rapid detection methods. In this study, a paper-based biosensor integrating a dual-recognition strategy using aptamers and antibodies was developed for the sensitive, rapid, and on-site detection of S. aureus in complex milk matrices. The biosensor combines a milk matrix-adapted aptamer with polyclonal antibodies (pAbs) and utilizes colloidal gold nanoparticles (AuNPs) as visual signal reporters. Using a SELEX process tailored to the milk matrix, the high-affinity aptamer SA2-1 was selected to specifically bind S. aureus, while pAbs enabled multi-epitope capture on the paper substrate. The aptamer–AuNP conjugates generated visual signals, achieving a detection limit of 102 CFU/mL within 15 min without an instrument. This dual-recognition strategy synergistically enhances both sensitivity and specificity, offering a cost-effective solution for dairy safety monitoring.
A novel biosensor employing dual-signal output was developed through the combination of efficient magnetic separation and dual-modal precise detection that can sensitively detect S. aureus in meat matrices, thus showing good application prospects in food matrices and further improving the reliability and specificity of...
Kanamycin (KANA), a widely used aminoglycoside antibiotic in animal husbandry, is associated with residue accumulation in foods due to improper use, threatening food safety. Detecting trace KANA in complex food systems such as dairy, meat, and apicutural products remains challenging because of matrix interferences (e.g...
This overview provides a design-oriented framework for nanoparticle-based biosensor selection in food safety by integrating nanomaterial function, biosensor design, and performance characteristics.
Sümeyra Savaş, Seyed Mohammad Taghi Gharibzahedi· Biosensors· 0 citations
Rapid on-site detection of bacterial contamination remains an urgent need in public health and environmental monitoring. Herein, we report a one-step, label-free colorimetric sensor for broad-spectrum bacterial detection based on a competitive electrostatic mechanism. Cationic poly-l-lysine (PLL) induces the aggregatio...
Rapid detection of pathogenic bacteria in water is essential for environmental monitoring and public health. Here, we present a duplex microfluidic fluorescence sensor based on bacteria-specific cell-imprinted polymer (CIP) thin films for simultaneous detection of Salmonella enterica and Escherichia coli O157:H7. The s...
Islam Mahmoud, Ali Doostmohammadi, P. Rezai· IEEE Sensors Letters· 0 citations
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