Effect of carbamazepine in pentylenetetrazole-induced epilepsy in rat model

Abstract

Purpose: To investigate the effects of carbamazepine on oxidative stress biomarkers and seizure-associated behavioral alterations in pentylenetetrazole (PTZ)-induced epilepsies.Method: A total of 15 adult male Wistar rats were randomly assigned into three groups (n = 5 in each group). Group 1 was the control group and received 0.9 % normal saline, group 2 received PTZ (40 mg/kg intraperitoneal), and group 3 received carbamazepine (CBZ; 50 mg/kg, oral gavage) 1 hour before PTZ administration for 14 days. Seizure latency, frequency, and duration were assessed. Oxidative stress biomarkers, including superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA), were determined in brain homogenates using standard spectrophotometric methods.Results: Pentylenetetrazole-induced changes in all measured parameters were significant at p < 0.001. However, following carbamazepine treatment, the increases in SOD and GSH were significant at p < 0.05, whereas the improvements in seizure latency, seizure frequency, seizure duration, and MDA were significant at p < 0.001.Conclusion: The study reveals that modulating oxidative stress may play a potential role in mediating the neuroprotective effects of CBZ, suggesting a promising therapeutic target in epileptogenesis.