Generation and characterization of two iPSC lines INDBi002-A and INDBi002-B from human keratinocytes of a healthy female using Sendai Virus reprogramming.

Abstract

We established two fully characterized induced pluripotent stem cell (iPSC) lines from human keratinocytes via Sendai virus-mediated reprogramming. This non-integrating approach maintains genomic integrity, facilitating the generation of pluripotent cell lines with stable self-renewal and multilineage differentiation potential. Characterization confirmed the expression of stemness markers, the capacity for trilineage differentiation, and a normal karyotype. The iPSC lines are a valuable platform for applications in disease modeling, pharmacological screening, and regenerative medicine.