Oct 2026· Current Directions in Biomedical Engineering· Vol 12, pp. 592 - 595· 0 citations· 5 references
Abstract
Abstract Cell spheroids provide physiologically relevant 3D cell models, but the manual handling of individual spheroids remains challenging, particularly in miniaturized, high-density formats such as droplet microarrays (DMAs). Here, we present ALPS, an automated liquid-handling platform that collects spheroid-containing nanoliter droplets and redeposits them to specified target spots within or between DMAs. Using HEK (human embryonic kidney) spheroids, ALPS achieved transfer yields of up to ~80% under optimized conditions with a processing time of ~5 s per sample. Transferred spheroids retained morphology after handling and showed a ~1.25-fold increase in diameter within 24 h, indicating that the transfer procedure did not compromise spheroid structural integrity. Together, these results establish ALPS as a practical interface between high-density on-DMA spheroid generation and standard downstream formats, including microtiter plates and tube racks, enabling scalable 3D culture workflows.
Perfused spheroid platforms enable continuous mass transport, but the role of chamber architecture in in situ spheroid assembly remains insufficiently understood. Here, we developed a perfusable organ-on-a-chip platform for the in situ formation and culture of HepG2 spheroids and compared rectangle-, rhombus-, and roun...
Juan Zhang, Hong-Ze Yin, Hui-Ying Yang et al.· ACS Biomaterials Science & E...· 0 citations
Oxygen (O2) transport is a major limitation in high-density cell culture, particularly as three-dimensional (3D) spheroids are increasingly used to better mimic in vivo tissue environments. Here, we developed a poly(4-methyl-1-pentene) (PMP)-based microwell platform, leveraging its lower molecular absorption and greate...
Jun Takahashi, Hiroyuki Kato, H. Zook et al.· bioRxiv· 0 citations
Tempo-spatially controlled culture and assembly of cellular spheroids are fundamental in engineering tissue-like assembloids for scalable application, yet remain a technical challenge. Here, we show self-buoyant culture of cell spheroids and programmable assembly of multi-spheroid by microbubbles in a high-throughput,...
Y.-J. Xiang, Bin-Qi Wei, Yue-Yang Sun et al.· Science Advances· 0 citations
Photothermal therapy (PTT) is a promising cancer treatment modality, but its evaluation remains limited by the lack of physiologically relevant and quantitatively controlled in vitro platforms. Here, we present a droplet-based microfluidic platform that enables single-spheroid formation, programmable nanoparticle deliv...
Hasti Honari, Léo Dancert, Caroline Parent et al.· ACS Applied Materials and In...· 0 citations
The intrinsic heterogeneity of solid tumors remains a crucial barrier to understanding cancer biology and predicting therapeutic outcomes. Here, we introduce a scalable and versatile biofabrication platform that enables the controlled assembly of multitype spheroid arrays (MSA) within standard culture microplates. By p...
Simone Bonetti, Eva Clemente, E. Mensà et al.· Advanced Healthcare Material...· 0 citations
Theranostic liposomes carry a drug and an imaging agent in the same particle, so that treatment and the means of watching it arrive together. Few have reached patients, and the obstacle is more often manufacturing than biology. Conventional bulk routes — thin-film hydration, ethanol injection, high-pressure homogenisat...
Unknown authors· Biosensors and Nanotheranost...· 0 citations
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