Multi-layer network biology combined with single-cell evidence reveals core regulatory nodes in atherosclerotic plaques and potential drug-targeting directions.

Multi-omics characterization of anoikis-related hub genes and macrophage-centered immune persistence with validation of HAVCR2 in atherosclerosis

Background Atherosclerosis (AS) is a chronic inflammatory vascular disease characterized by immune dysregulation, metabolic disturbance, and progressive vascular remodeling. Although anoikis resistance has been extensively investigated in cancer biology, its involvement in immune-cell persistence and plaque progression...

Single-Cell and Machine Learning Analyses Identify a PFKFB3-Centered Regulatory Network and Potential Salidroside Interaction in Coronary Heart Disease

The findings support PFKFB3 as a potential biomarker and mechanistic hub and suggest that salidroside may modulate its activity and provide a multi-layered framework connecting glycolytic remodeling, inflammatory transcriptional activity, and intercellular signaling in CHD.

Identification of Key Genes and Pathways in Parkinson’s Disease Through Bioinformatics Analysis

Integrative transcriptomics identified conserved central-peripheral signatures in PD and five hub genes are candidate biomarkers/therapeutic targets, offering a systems-level framework for disease mechanisms and independent cohort validation.

Identification of Candidate Biomarkers and Signaling Pathways Associated with Alzheimer’s Disease Using Bioinformatics Analysis of Next Generation Sequencing Data and Molecular Docking Studies

The GO and pathway enrichment analysis results revealed that the up regulated genes were mainly enriched in response to stimulus, cytoplasm, small molecule binding and signal transduction, whereas down regulated genes were mainly enriched in multicellular organism development, cell junction, ion binding and cardiac con...

Machine learning and single-cell RNA sequencing identify shared diagnostic genes and mechanistic links between antiphospholipid syndrome and carotid atherosclerosis

Background Antiphospholipid syndrome (APS) is an acquired autoimmune disorder characterized by recurrent vascular events in large, medium, or small vessels. These events contribute to cardiovascular disease primarily through thrombosis and atherosclerosis (AS). Carotid atherosclerosis (CAS) represents a particularly hi...

Screening for candidate genes and cell populations associated with atherosclerotic calcification using single-cell RNA sequencing