Aug 2026· Scientific Reports· Vol 16· 0 citations· 27 references
Medicine
TL;DR
A comparative evaluation of two distinct artificial intelligence paradigms for the analysis of optical microscope images of microplastics shows that the trained DL model excels in high-throughput, reproducible screening, while the VLM-HITL system offers enhanced interpretability and flexibility for ambiguous cases.
Abstract
Microplastic (MP) pollution poses escalating environmental risks, demanding efficient and reproducible tools for morphological characterization of plastic particles. Traditional manual microscopy is labour-intensive, operator-dependent, and poorly suited to large-scale monitoring. This study presents a comparative evaluation of two distinct artificial intelligence paradigms for the analysis of optical microscope images of microplastics. The first paradigm is a domain-specific, multi-task deep learning (DL) classifier based on EfficientNet-B0 with transfer learning, trained on an in-house dataset of approximately 700 annotated microscope images to simultaneously predict microplastic shape/type (five classes), color (10 classes), and surface texture (two classes). The second paradigm employs the Claude Vision API as a zero-shot vision–language model (VLM), augmented with a structured human-in-the-loop (HITL) mechanism allowing domain experts to provide targeted guidance for ambiguous particles. Both systems were evaluated on an identical, independent test set using accuracy, macro-averaged precision, recall, and F1-score. The DL classifier achieved F1-scores of 91.2%, 88.5%, and 85.1% for shape/type, color, and texture classification, respectively. In contrast, the VLM achieved raw F1-scores ranging between 72 and 81% across the evaluated tasks, which improved substantially to approximately 84–89% following expert-guided refinement. These results demonstrate that the trained DL model excels in high-throughput, reproducible screening, while the VLM-HITL system offers enhanced interpretability and flexibility for ambiguous cases. This comparative framework, deployed as a freely accessible web application via Hugging Face Spaces, provides practical insights into the deployment trade-offs between domain-specific and generalist AI approaches for environmental microplastic analysis.
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