Application of LC-MS/MS for Monitoring Mycotoxin Contamination in Wildlife Feed: A Case Study from Imam Turki bin Abdullah Royal Reserve

Abstract

Several fungus produce secondary metabolites called mycotoxins, which are known to have significant adverse impacts on both human and animal health. Many diseases may result from the toxicity of animal feed contaminated with mycotoxins. This work used LC-MS/MS to evaluate the feeds for residual mycotoxins, aflatoxins (AFs) B1, B2, G1, G2, ochratoxin A (OTA), zearalenone (ZON), and fumonisin B1 simultaneously. According to the findings of the LC-MS/MS analysis, none of feed and forage (alfalfa) samples contained aflatoxins (B1, B2, G1, or G2). Additionally, no OTA was found in any of the samples. Zearalenone was detected in Feed 1 and Feed 2 samples at quantities of 0.112µg/g and 0.103µg/g, respectively. The amounts of fumonisin B1 in Feed 1 and Feed 2 samples were 71.24µg/g and 62.23µg/g, respectively. However, Forage 3 and Forage 4 did not detect it. After year quarter analysis were repeated on number of samples. AFB1 was found in Feed of the samples, but other types of AFs, OTA and ZON were not detected. Fumonisin B1 was appeared in safe level at feed samples. All toxins were absent from all forage samples. Proximate analysis of samples has shown that it is rich in carbohydrates, protein, and fiber. Mineral analysis has shown that Calcium and Magnesium levels around 1 and 0.4% respectively. This approach will make it easier to control and analyze mycotoxins in feed in a lab. Because of its multidetector capabilities, LC-MS/MS is the most widely used analytical technique for approaches.