Study of the protective effect of alcoholic extract of flax seeds against thioacetamide-induced hepatotoxicity in male albino rats

Abstract

The current study aims to investigate the role of flaxseed extract versus hepatotoxicity stimulated by thioacetamide in male white rats by examining some modifications. Liver tissue was examined for enzymes and antioxidants, urea, and creatinine. In this experiment, 25 male albino rats were divided into five groups: the control group (G1) and the treatment group (G2). They were injected with thioacetamide at a dose of 300 mg/kg for a month. The third, fourth, and fifth groups (G3, G4, G5) were dosed daily with the alcoholic extract of flax seeds at doses of 400 mg/kg, 600 mg/kg, and 800 mg/kg, respectively, and then injected with thioacetamide (300 mg/kg), while continuing to be dosed with the alcoholic extract of flax seeds. The samples of tissue and blood were collected after completion of the experiment, and changes in liver tissue, liver enzymes (AST, ALT, ALP, LDH), antioxidants (MDA, GSH), urea, and creatinine were measured. The results showed changes in liver tissue with an increase in the levels of liver enzymes (AST, ALT, ALP, LDH), malondialdehyde, urea, and creatinine, and a decrease in the level of glutathione after treatment with thioacetamide. Treatment with the alcoholic extract of flax seeds led to a significant improvement in liver tissue, a decrease in the levels of liver enzymes, malondialdehyde, urea, and creatinine, and an increase in the level of glutathione. We conclude that the alcoholic extract of flax seeds plays a protective role against hepatotoxicity resulting from treatment with thioacetamide.