Bioactive Injectable Brushite Cement Reinforced With Platelet‐Rich Plasma for Bone Regeneration

Abstract

Injectable bone substitutes (IBSs) made from brushite‐calcium phosphate are promising biomaterials for bone tissue regeneration, offering favorable setting kinetics and excellent biocompatibility. However, relatively poor mechanical properties limit their application primarily as filler materials in orthopedic and craniofacial regeneration process. In the present study, brushite cement composition was modified using monocalcium phosphate monohydrate (MCPM) and biphasic calcium phosphate (BCP) to improve stability. Physicochemical, mechanical, and in vitro biological properties were systematically evaluated, revealing that the properties of IBS are influenced by the MCPM concentration, with a BCP:MCPM ratio of 50:50 indicating the optimized formulation for injectable bone cement. Further, to enhance the osteoinductivity of IBS, platelet‐rich plasma (PRP) was incorporated in the composite. Experimentally, both in vitro as well as in vivo studies confirmed that PRP‐integrated IBS promotes appreciable bone regeneration. Overall, the results indicate that PRP‐integrated IBS holds significant promise as an injectable biomaterial for rapid bone regeneration.