Trichoderma spp., Bacillus spp. and Pseudomonas spp. isolated from commercial products show biocontrol activity against soil-borne pathogens in soybean
Aug 2026· Scientific Reports· Vol 16· 0 citations· 68 references
Medicine
TL;DR
Overall, in planta efficacy was highly variable compared to the strong in vitro inhibition, Nevertheless, selected commercial strains show clear potential to mitigate severe disease symptoms under high R. solani pressure.
Abstract
This study evaluated the biocontrol potential of ten commercially available microbial soil additives and fungicides against major soil-borne pathogens (Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum and Diaporthe longicolla) to identify immediate, market-ready solutions for farmers, in the case of soil additives without the delay of product registration. In vitro dual cultures demonstrated strain- and pathogen-dependent antagonistic activity, with Pseudomonas and Bacillus being less effective than the tested Trichoderma strains. Regarding bacterial antagonists, the production of volatile organic compounds (VOCs) and lytic enzymes was confirmed in vitro, indicating potential mechanisms of antagonism. Compatibility tests on soybean plants demonstrated no negative effects on germination, overall growth, or nodule abundance in nearly all cases. In greenhouse bioassays, under high R. solani pressure (1.5%) microbial treatments did not significantly restore germination rate and seedling growth. However, T. harzianum T50 (Vitalin Trichoderma) and T. harzianum + P. fluorescens mixture (Trillus), significantly reduced hypocotyl lesion severity and increased the proportion of symptom-free seedlings. Overall, in planta efficacy was highly variable compared to the strong in vitro inhibition. Nevertheless, selected commercial strains show clear potential to mitigate severe disease symptoms under high R. solani pressure.
Findings highlight the potential of indigenous rhizosphere microorganisms as biological control agents for the management of wheat root rot pathogens and identify the Bacillus strain showing the strongest growth-promoting effect.
N. Kuldybayev, A. Sadanov, G. Baimakhanova et al.· Brazilian Journal of Biology· 0 citations
The Streptomyces strain Strep_22 represents a highly promising, dual-action biocontrol agent that effectively protects wheat and tomato crops against devastating fungal infections while simultaneously stimulating plant vegetative growth and development.
Thomas Conte, Maria Grazia Morea, Gaetana Ricciardi et al.· Pest Management Science· 0 citations
Agricultural residues rich in cellulose remain
underutilized, while soil-borne pathogens continue to
threaten crop productivity. This study isolated and
characterized native Bacillus and Trichoderma strains
from Vietnamese soils to evaluate their cellulolytic
capacity, antifungal potential and effects on mustard
greens (Brassica juncea). Bacillus isolates were
identified by 16S rRNA sequencing whereas
Trichoderma strains were distinguished based on
morphological and microscopic traits. Cellulase
activity was assessed on carboxymethyl cellulose agar
and antagonism against Neoscytalidium dimidiatum.
Pythium sp. was tested through dual-culture assays.
Among the isolates, Bacillus amyloliquefaciens and
Trichoderma harzianum exhibited the strongest
cellulolytic activity and fungal inhibition. Greenhouse
trials further demonstrated that inoculation with these
strains, individually or in combination, enhanced plant
biomass and reduced disease symptoms under
pathogen challenge.
The results highlight the dual role of indigenous
microbial inoculants in valorizing lignocellulosic
waste and providing eco-friendly biocontrol. Such
dual-function inoculants offer a sustainable alternative
to chemical fungicides while promoting circular
resource use in Vietnamese vegetable production
systems.
My-Phi-Long Nguyen, Tran-Thuy-Thanh-Mai Nguyen, Thuy-Vy-Ha Bui et al.· Research journal of biotechn...· 0 citations
Trichoderma
species are capable of causing significant changes in the metabolic activities of host plants, thereby triggering plant growth and increasing plant defense mechanisms against a diverse variety of fungal infections. This species has been reported as potential bio-control agents of many phytopathogenic fungi. Therefore, this study aimed to assess the
in vitro
antagonistic activity of indigenous
Trichoderma
spp. isolated from selected locations across Kano State, Nigeria, against phytopathogens affecting selected vegetables, in a dual-plate assay. Molecular characterization revealed three
Trichoderma
isolates:
T. koningii
(accession number KY886143.1; 99.63% BLAST similarity),
T. harzianum
(KJ807357.1; 98.94%), and
T. viride
(OP546802.1; 98.12%). The results revealed that
Trichoderma
spp. inhibited the radial growth of isolated phytopathogenic fungi compared to the control. The percentage growth inhibition for
T. harzianum
was higher in the tested species. Specifically, the inhibition percentages of
T. harzianum
ranged from 10.82 to 72.32%, for
T. koningii
from 5.49 to 63.15%, and for
T. viride
from 2.22 to 47.33% in the dual-culture assay. The results for
Trichoderma
spp. showed a significant decrease in radial growth and a statistical difference (
p
< 0.05) in the percentage inhibition of the tested pathogens using a one-way ANOVA. The results of this study reveal valuable knowledge on the mycoparasitic potential of
Trichoderma
spp. that could be used in the selection of suitable biological control agents. They also provide useful insights into phytopathogen interactions, relevant to developing strategies for the biological control of fungal phytopathogens in crops of agricultural importance.
Unknown authors· Frontiers in Microbiology· 0 citations
Postharvest soft rot is a major constraint in kiwifruit production, causing substantial economic losses and raising food safety concerns. Therefore, the development of sustainable biocontrol alternatives to conventional chemical treatments is urgently needed. In this study, thirty-seven fungi were isolated from healthy kiwifruit tissues and rhizosphere soil and screened for their biocontrol potential. Three highly effective antagonistic strains were ultimately identified: Mucor circinelloides N2-1-1, Aspergillus niger Pb-2-2, and Trichoderma hamatum Nd-1-1. In vitro antagonism assays showed that A. niger Pb-2-2 exhibited the strongest and broadest-spectrum inhibitory activity against four postharvest pathogens, namely Fusarium sp., Alternaria sp., Botryosphaeriaceae sp., and Phomopsis sp. In contrast, in vivo protection assays demonstrated that T. hamatum Nd-1-1 almost provided the highest control efficacy against all four pathogens in kiwifruit fruit. Fermentation broth protection experiments further revealed that A. niger Pb-2-2 produced stable and broad-spectrum antimicrobial metabolites. Although M. circinelloides N2-1-1 showed the weakest antagonistic activity among the three fungi, this is the first report indicating that M. circinelloides has antagonistic activity against phytopathogenic fungi. Physiological analyses indicated that all three antagonistic fungi alleviated the inhibitory effects of pathogens on superoxide dismutase, catalase, and ascorbate peroxidase activities, helped maintain reactive oxygen species homeostasis, and activated the phenylpropanoid pathway, thereby enhancing disease resistance in kiwifruit. These findings provide a theoretical basis and promising microbial resources for the biological control of postharvest soft rot in kiwifruit.
Ji-Qing Lei, Hong Li, Yin-Na Shi et al.· Journal of Fungi· 0 citations
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