THE EFFECT OF NATURALLY OCCURRING CHELIDONIC ACID ON THE MORPHOFUNCTIONAL STATUS OF INTERNAL ORGANS : AN EXPERIMENTAL STUDY

Abstract

For the first time, a comprehensive in vivo evaluation of the effects of long-term (45 days) daily intragastric administration of water solution of naturally occurring small molecules of chelidonic acid (ChA) to Balb/c mice was conducted. The study was focused on the morphofunctional state of several key organs involved in xenobiotic metabolism and excretion, including the stomach, liver, kidneys, as well as an immunocompetent organ (spleen). The obtained results demonstrate the absence of pathological changes in these organs when the substance was administered orally at a concentration of 10 mg/ kg in 0.2 ml of solvent (distilled water) for 45 days. Moreover, as previously established, ChA and its calcium salt at the indicated dose exhibit specific (osteogenic) activity against mesenchymal stromal/stem cells both in vitro and in vivo. These findings indicate an absence of general toxicity to studied organs of a low concentration of ChA in lineage mice, suggesting that it may be a promising candidate for future clinical use. This opens up promising opportunities for further research and development of an active pharmaceutical substance that is entirely domestic in origin. This is of great importance for the advancement of the field of regenerative biomedicine, primarily in the area of bone tissue engineering.