Exchange dynamics and kinetic control of gene regulation complexes.

Modulation of noisy gene expression by general sequestration mechanisms

Sequestration of gene products through diverse mechanisms forms a fundamental layer of regulation in intracellular biochemical processes, including post-translational modification, promiscuous binding to genomic decoy sites, and partitioning into membraneless compartments formed through phase separation. Here, we devel...

Characterization of RNA Structure Dynamics with Trans-Factors Using Single-Molecule Optical Tweezers.

Quantitative Model of Transcriptional Noise Regulation by mRNA Condensates

This work develops a minimal theoretical framework for quantitative investigation of the role of reversible mRNA condensation in transcription, and elucidates important microscopic aspects of transcription, providing a convenient quantitative tool for investigating complex biological phenomena.

Integrative structural biology of protein-RNA interactions in four dimensions.

Recent developments in integrative modeling of protein-RNA complexes are reviewed, highlighting advances in in-cell, 4D and condensate structural biology, and how these approaches shape the understanding of RNP assembly, regulation, and function in physiologically relevant environments are discussed.

Current Challenges of Transcription Compartmentalization Research

The clearest evidence for a functional role of condensation may emerge in contexts where transcription must generate switch‐like responses, such as cell‐fate decisions, stress responses, environmental sensing, and disease.

High-throughput methods for studying protein self-assembly.

Emerging high-throughput strategies to study protein condensation and aggregation at scale are reviewed, emphasizing what they truly measure, their limitations, and how the cross-talk among these complementary approaches can provide a more accurate and mechanistic mapping of sequence-to-assembly relationships.