TL;DR
Fluorescence observation analysis indicated that the genetic stability of cloned genes was markedly improved by the Bacmid variant-derived baculoviruses with sustained expression for up to 20 passages, appropriately 2–3 times longer than the wild-type control.
This engineering method allows for endogenous expression of the Soc-mCherry recombinant protein within Escherichia coli cells during phage replication, after which the recombinant protein spontaneously assembles onto the capsid of the engineered phage.
Beauveria bassiana is a filamentous fungus that has considerable potential to combat insect pests. Therefore, an easy to use genetic transformation tool helps to investigate which fungal genes play a role in virulence and host specificity. However, B. bassiana is often very resistant to antibiotics used as selective ag...
A 140% increase in pHelper plasmid production is reported, while the most successful ITR variant increased pTransgene plasmid yields by 57% and transgene expression by 28%, without reducing rAAV yields or transduction efficiency.
It is demonstrated that a potent constitutive promoter system and a hammerhead ribozyme enhance the efficiency of positive-sense RNA virus rescue using CPER, and may support applications requiring efficient recovery of low fitness mutants and diverse mutational libraries and barcoded virus populations.
High-yield recombinant protein expression is a critical requirement in biopharmaceutical development. The pET vector family is among the most widely used systems for protein production in Escherichia coli. Shilling et al. (2020) reported that insertion of a GAGA sequence within the T7lac promoter region of pET28a enhan...
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