Findings suggest that the synthesized hydrogel (BSG‐CHI) provides a favorable microenvironment for tissue regeneration and wound management applications.
Abstract
Chronic wounds pose a major clinical challenge due to a complex pathophysiological condition. It is characterized by persistent inflammation, excessive oxidative stress, and a high risk of bacterial infection, which hinders tissue repair. To address this, a multifunctional hydrogel dressing based on gallic acid conjugated chitosan (G‐CHI) and integrated with simvastatin and heparin‐functionalized Ag/Zn‐doped bioactive glass (HBG). The incorporation of gallic acid improved the structural integrity and viscoelastic behavior of the hydrogel. The release kinetics study demonstrated controlled liberation of Ag
+
and Zn
2+
ions within biologically safe limits over 24 h, while simvastatin exhibited diffusion‐controlled release behavior. The hydrogel also exhibited desirable adhesive properties, indicating its suitability as a wound dressing material. In vitro biofunctional assays showed enhanced antioxidant activity (~75% free radical scavenging), hemocompatibility (< 5% hemolysis), and effectiveness against both
Staphylococcus aureus
and
Escherichia coli
(> 90% efficacy in time kill assay). Further, synergistic release of simvastatin and bioglass showed enhanced proliferation of fibroblast (NIH 3T3), keratinocyte (HaCaT), and endothelial cells (SVEC), accompanied by increased collagen deposition compared to control groups. These findings suggest that the synthesized hydrogel (BSG‐CHI) provides a favorable microenvironment for tissue regeneration and wound management applications.
With its integrated hemostatic, antioxidant, antibacterial, and pro-regenerative properties, the CBOS hydrogel offers a viable and attractive therapeutic approach for complex wound tissue repair.
Xueyan Hou, Yanan Lu, Tenglong Xu et al.· ACS Applied Materials and In...· 0 citations
Stable hydrogel formation with favorable porous architecture, swelling behavior, thermal stability, and homogeneous distribution of nanovesicles was well established and exhibited excellent biocompatibility, hemocompatibility, and enhanced cell-material interactions.
Rizos Evangelos Bikiaris, Ioanna Koumentakou, A. Niti et al.· ACS Applied Bio Materials· 0 citations
Chronic diabetic wounds remain a major clinical challenge owing to persistent bacterial infection, prolonged inflammation, excessive exudation, and impaired tissue regeneration. Herein, an injectable thermosensitive hydrogel was developed by integrating N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan chloride with aldehyde-functionalized Pluronic F127 for epidermal growth factor (EGF) delivery and diabetic wound repair. The hydrogel forms a dual-crosslinked network through temperature-induced micellization and dynamic Schiff base bonding, exhibiting rapid gelation under physiological conditions, shear-thinning behavior, and self-healing properties. In vitro, the hydrogel provides a sustained release profile of EGF exhibiting effective antibacterial activity against Gram-positive S. aureus. In vivo studies in streptozotocin-induced diabetic rats demonstrate significantly accelerated wound healing, achieving 83% wound closure within 14 days compared to 45% in the control group, along with enhanced tissue regeneration characteristics, including improved collagen deposition. This multifunctional hydrogel provides a promising strategy for diabetic wound management by integrating antibacterial potential and tissue regeneration.
A multifunctional hydrogel patch developed by chemically modifying chitosan with N-acetylsulfonyl chloride and forming a cross-linked network with polyvinylpyrrolidone (PVP) represents a promising multifunctional dressing for the effective management of infected wounds.
Insha Kakroo, Nayeema Gull, Insha Mehraj et al.· ACS Applied Bio Materials· 0 citations
Topical delivery systems represent a promising strategy to reduce adverse effects associated with conventional therapies devoted to the treatment of skin disorders. This study deals with the fabrication of a biopolymeric platform for topical delivery of morin (M), a bioactive flavonoid with antioxidant and anticancer properties. Hydrogels based on gelatin (G), chitosan (Ch), and arabic gum (AG) were synthesized by freeze–thaw method. M was loaded to impart the therapeutic action. Characterization of hydrogels demonstrated that Ch incorporation improved mechanical and thermal properties, swelling, and water stability. Two loading methods were explored to incorporate M: surface adsorption and drug encapsulation during gel formation. The reached efficiencies were 42% and 100% for adsorption and encapsulation, respectively. Antioxidant activity, evaluated by scavenging DPPH radicals (%), was also enhanced by M loading reaching 45% versus 22% when the raw hydrogel was assayed. In vitro cytotoxicity studies confirmed the biosafety of formulations. Drug release experiments showed that 65% of M was released from hydrogel after 120 min in a skin‐simulating medium. Molecular simulations were performed to justify the M retention. The achieved findings highlight the critical role of molecular‐level interactions in governing M release, providing guidelines for the development of hydrogel‐based topical delivery systems.
Ana Karen Jaimes, F. Favatela, Paula Nicolás et al.· ChemMedChem· 0 citations
Diabetic wound healing poses a significant clinical challenge due to high blood glucose, infection susceptibility, and impaired regeneration. However, current film‐based hydrogel dressings often have limited gas permeability that restricts oxygen diffusion and may cause inflammation with prolonged use. This investigation presents an electroactive sodium alginate (SA)‐based hydrogel textile through the synergistic effect of tannic acid (TA)‐mediated carbon nanotubes (CNTs) dispersion and antibacterial properties. The dressing exhibits a skin‐mimicking electrical conductivity of 0.107 S/m and remarkable broad‐spectrum antibacterial activity, achieving an inhibition rate of over 99% against both Escherichia coli (E. coli) and methicillin‐resistant Staphylococcus aureus (MRSA). Significantly, its antibacterial effect is achieved without relying on photothermal effects or antibiotic release, effectively avoiding thermal injury and drug resistance risks. In vivo experiments demonstrate that the hydrogel dressing can notably decrease the level of the pro‐inflammatory cytokine IL‐1β at the wound site, stimulate collagen deposition, and accelerate epithelialization. As a result, it enables over 99% closure of diabetic wounds within 18 days. This woven dressing provides a promising new design and preparation strategy, holding great potential for improving the clinical treatment of diabetic wounds.