TL;DR
It is found that histone modifications do not occur independently; there are roughly two groups of co-occurring modifications that are consistent with the idea of a simple, redundant histone code, in which multiple modifications share the same role.
This chapter describes the experimental setup and provides a detailed protocol for performing chromatin immunoprecipitation in Ustilago maydis or related smut fungi.
The six-residue loop 2 of H2A and H2A is used to map, at single-residue resolution, how nucleosome-core variation reshapes cellular function, and identifies histone variation as a source of phenotypic innovation.
Nucleosome positioning plays a fundamental role in regulating DNA accessibility and cellular activity, yet the precise rules by which mammalian sequences instruct this organization have remained incomplete. Prior work has established that intrinsic sequence preferences dictate nucleosome architecture in yeast, but in m...
Nucleosomes integrate diverse histone variants to organize chromatin and coordinate genome maintenance. In plants, the structural interplay between replication-associated histones and DNA damage-responsive histone variants remains poorly understood. Here, we determine cryogenic electron microscopy structures of an Arab...
Alterations in histone acetylation are frequently detected in various cancers and neurological diseases. Histone acetylation is known to disrupt both intra- and inter-nucleosomal interactions, but its influence on higher-order chromatin organization has been studied to a lesser extent. In this study, we changed global...
HisTrader is presented, a computational approach that identifies nucleosome-free regions (NFRs) within ChIP-based profiling of histone modification peaks, which reduces the target sequence length for motif discovery and genetic variant prioritization.
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