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Open access Aug 2026

GTX-GUT: A Standardized Metagenomic Workflow for Gut Microbiome Profiling and Clinical Associations

Application to a human sample from a patient with type 2 Diabetes Mellitus recovered a dysbiotic signature consistent with the literature, including reduced Firmicutes abundance, elevated Bacteroidetes and Proteobacteria, and a predominance of clinical associations within metabolic and gastrointestinal categories.

Rodrigo Lima Andrade, Tayná da Silva Fiúza, J. Kroll et al. · 0 citations
Open access Sep 2026

Metax enables accurate cross-domain taxonomic profiling of metagenomes.

This broad evaluation demonstrated that Metax resolved bacterial and viral signatures of peri-implantitis in oral microbiomes and revealed signals suggestive of reagent-borne contaminants and reference misassemblies in plasma-cell-free DNA.

Zhi-Luo Deng, Nasim Safaei, A. McHardy · 0 citations
Open access Aug 2026

Development and Validation of Mock Communities as Quality Control Materials for Clinical Metagenomic Next-Generation Sequencing.

Four types of bead-based mock communities comprising 26 species commonly found in the gastrointestinal tract, respiratory tract, skin, and genital tract plus cerebrospinal fluid are developed and are essential QC materials for clinical metagenomic NGS analysis.

Dokyun Kim, Songhee Lee, Da-Young Kang et al. · 0 citations
Review Open access Jul 2026

From Scat to Sequencing: Non-Invasive Methods for Wildlife Microbiome Research and Conservation

Current non-invasive sampling methods and advanced molecular tools for microbial profiling are discussed, emphasizing the integration of microbiome research into conservation strategies to strengthen biodiversity preservation amid ongoing environmental challenges.

Pritee Chunarkar, Manoj Malavika, Rahul Gupta et al. · 0 citations
Review Open access Jul 2026

Methodological bias shapes the interpretation of the urinary microbiome in low-biomass systems

How factors such as sample collection methods, contamination dynamics, DNA extraction protocols, sequencing approaches, and bioinformatic pipelines shape microbial profiles and contribute to inconsistencies in microbiome–disease associations is examined.

Viviana Méndez-Sacta, Solmayra Agreda Orellana, Luz María Ayavaca-Tapia et al. · 0 citations
Open access Sep 2026

Seven gut microbiome types across two Japanese cohorts defined by co-abundance-based microbial guilds and associated with host physiology

Collectively, co-abundance-defined microbial guilds capture coherent ecological and functional states of the gut microbiota, providing a transferable framework for linking gut microbiome organization to host physiology beyond conventional taxonomy-based classification.

Jiro Nakayama, Misako Yoden, Ayaka Uchikawa et al. · 0 citations

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