Complete genome sequence of Idiomarina sp. D18 from the Western Pacific Ocean reveals biosynthetic potential for β-lactone and RiPP-like compounds.

Complete genome sequence of a Methylomonas sp. nov. 2F7 isolated from rice paddy soil in South Korea.

OBJECTIVE This Data Note reports the complete genome sequence and associated functional-genomic data of Methylomonas sp. strain 2F7, a methane-oxidizing bacterium isolated from rice paddy soil in South Korea. These data were generated to support comparative genomic analyses of methanotrophic bacteria and to document the genome-based taxonomic position and C1 metabolism gene repertoire of strain 2F7. DATA DESCRIPTION The dataset includes Oxford Nanopore Technologies MinION raw reads, a two-replicon genome assembly, annotation summaries, functional classifications, a phylogenetic analysis, average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons, and a curated C1 metabolism gene table. The genome consists of a 4,979,984-bp chromosome and a 23,603-bp plasmid, with an overall G+C content of 51.4%. Annotation identified 4,520 genes, including 4,429 coding sequences (CDSs), nine rRNA genes, 46 tRNA genes, and four ncRNAs. The 16S rRNA gene sequence showed the highest similarity to Methylomonas fluvii EbBᵀ, whereas ANI/dDDH analysis identified Methylomonas rosea WSC-7ᵀ as the closest related type strain by genome-distance criteria, with ANI/dDDH values of 89.6% and 38.0%, respectively. Additional ANI/dDDH comparisons with M. fluvii EbBᵀ and M. defluvii OY6ᵀ yielded values of 89.3%/37.4% and 89.4%/36.9%, respectively. These genome-based values fall below accepted species-boundary thresholds and support placement of strain 2F7 as a putative novel species within the genus Methylomonas. The curated gene table documents pMMO-like and sMMO gene clusters and genes involved in methanol oxidation, formaldehyde oxidation, and ribulose monophosphate (RuMP)-linked C1 assimilation.

First Draft genome sequence of strain MF0224, representing an unclassified Fusobacterium-related lineage isolated from coastal sediments of Puducherry, India

This article presents the draft genome sequence of strain MF0224, an anaerobic bacterium representing an unclassified Fusobacterium-related lineage isolated from a coastal sediment sample collected in Puducherry, India. Whole-genome sequencing was performed using the Illumina paired-end sequencing platform and assembled into a draft genome of 2.02 Mb comprising 26 contigs with a genomic DNA G+C content of 28.19%. Genome annotation predicted 1889 coding sequences, 47 tRNA genes, and multiple genes associated with anaerobic carbon metabolism, acetate production, sulfur metabolism, volatile fatty acid metabolism, and carbohydrate-active enzymes. Genome quality assessment indicated high completeness with low contamination. Comparative genomic analysis using TYGS identified the strain as a potential novel species within the genus Fusobacterium, supported by low digital DNA–DNA hybridization (dDDH) values (∼20%) relative to currently described species. Average amino acid identity (AAI; 68.9%) supported its placement within the genus Fusobacterium while indicating substantial genomic divergence. Comparative genomic analysis also determined a percentage of conserved proteins (POCP) value of 65.9%, providing additional genomic characterization of strain MF0224. Phylogenetic analyses based on 16S rRNA gene sequences and whole-genome phylogeny consistently positioned strain MF0224 within the Fusobacterium-associated clade. The genome provides a valuable resource for investigating anaerobic carbon metabolism, acetate biosynthesis, sulfur-associated pathways, and the ecological adaptation of Fusobacterium-related bacteria in coastal sediment ecosystems.

Genome analysis of the glycosphingolipid-producing green alga Tetraselmis sp. NKG400013.

Microalgae are gaining attention as sustainable resources for the production of valuable compounds, including biofuels, pigments, and bioactive metabolites. To support metabolic engineering and genome editing approaches aimed at enhancing these traits, high-quality genome assemblies are essential; however, genomic information remains limited for many microalgal lineages. Tetraselmis sp. NKG400013 is a green alga known for high glycosphingolipid accumulation with distinctive structural features. Here, we report a draft genome assembly of this strain generated using PacBio HiFi sequencing and transcriptome-supported annotation. The assembled genome spans 423.7 Mbp, with 74.5% repetitive sequences and 15,322 predicted protein-coding genes. Comparative analyses across 11 green algal species revealed a positive correlation between genome sizes and repeat contents, indicating that transposable element expansion, particularly long terminal repeat retrotransposons, has substantially contributed to genome enlargement in Tetraselmis. Genome-wide functional annotation and ortholog inference identified core enzymes required for glycosylceramide biosynthesis. Both sphingolipid Δ4 and Δ8 desaturases were identified in Tetraselmis. and their coexistence suggests an expanded capacity for long-chain base modification that may underlie its distinctive glycosphingolipid profile. These results establish a genomic framework for understanding the high glycosphingolipid-producing capacity of NKG400013 and provide insights into the evolutionary diversification of sphingolipid metabolism in green algae.

Genomic exploration of Serratia rubidaea MJ24 uncovers diverse biosynthetic capacity and agricultural relevance

Complete genome sequence and mass spectrometry assist mining siderophore turnerbactin analogs in a non-model Pseudoduganella strain with biocontrol potential

Findings indicate that Pseudoduganella sp.

Whole-genome sequencing reveals adaptive strategies of the Antarctic aquatic bacterium Arthrobacter sp. R5.

Arthrobacter species are widely distributed in cold environments and are recognized for their ability to withstand multiple environmental stressors. Here, we report the whole-genome sequence of Arthrobacter sp. R5, a red-pigmented and UV-resistant bacterium isolated from shallow lake sediments collected at Edmonson Point, Antarctica. Whole-genome sequencing was performed using the Illumina NovaSeq 6000 platform, and the genome was assembled and annotated using the TORMES workflow. The assembled genome comprises 4,145,062 bp with a GC content of 67.78% and contains 3700 predicted coding sequences, 57 tRNAs, three rRNA genes, and one tmRNA gene. Functional classification based on Clusters of Orthologous Groups (COG) revealed a predominance of genes involved in transcription, amino acid and carbohydrate metabolism, signal transduction, and replication, recombination and repair. The genome harbours several genes associated with DNA damage repair, including photolyases, the UvrABC nucleotide excision repair system and the UmuCD SOS response system, which may contribute to the UV resistance phenotype of strain R5. Genome mining further identified multiple biosynthetic gene clusters, including terpene-associated regions containing genes related to carotenoid biosynthesis, supporting the genetic potential underlying the characteristic red pigmentation of this strain. The genomic dataset presented here provides a valuable resource for investigating the molecular mechanisms of environmental adaptation in Antarctic bacteria and for exploring the biotechnological potential of stress-protective metabolites produced by polar microorganisms.