Chromosome-Level Genome Assembly and Annotation of the Chinese Lizard Gudgeon (Saurogobio dabryi)
TL;DR
This study constructed the first chromosome-level genome assembly of S. dabryi by integrating PacBio HiFi long-read sequencing, short-read sequencing, and Hi-C chromatin interaction technology and annotated repetitive sequences, protein-coding genes, and non-coding RNAs, and explored its phylogenetic position within Cyprinidae.
Abstract
Simple Summary The Chinese lizard gudgeon (Saurogobio dabryi) is a commercially important benthic freshwater fish endemic to East Asian river basins, with high ecological and aquaculture value. However, the lack of a high-quality reference genome has limited research on its adaptive evolution, genetic breeding, and wild population conservation. In this study, we constructed the first chromosome-level genome assembly of S. dabryi by integrating PacBio HiFi long-read sequencing, short-read sequencing, and Hi-C chromatin interaction technology. The final assembly spans 1.09 Gb with 25 chromosomes, and exhibits high continuity, completeness, and accuracy. We further annotated repetitive sequences, protein-coding genes, and non-coding RNAs, and explored its phylogenetic position within Cyprinidae. This genomic resource provides a fundamental basis for future studies on benthic fish adaptation, germplasm improvement, and biodiversity conservation of Gobioninae species.