Discovery and Comprehensive Characterization of Pseudomonas sp. MUP55: Taxonomy, Massetolide-Mediated Biocontrol, and Regulatory and Antimicrobial Contributions of the pvf Cluster

Interactions Between Bacillus atrophaeus 100 MTN1 and Fusarium oxysporum f. sp. lycopersici Reprogram the Transcriptomic and Metabolomic Profile to Combat Tomato (cv. Kalyan) Wilt

This research evaluated the biocontrol potential of the bacterial flora from cured sugarcane bagasse (SCB) against Fusarium oxysporum f. sp. lycopersici (Fol), the causal agent of tomato Fusarium wilt. Screenings of twenty SCB-derived isolates revealed consistent antagonistic activity, inhibiting mycelial growth from 32.08% to 55.00%. The most effective isolate, 100MTN1, was identified via 16S rRNA sequencing (GenBank: PX506225) as Bacillus atrophaeus. Interaction between B. atrophaeus 100MTN1 and Fol FOLViF has revealed a distinct profile of bioactive metabolites produced specifically during co-cultivation. Transcriptomic profiling of Fol FOLViF exposure to 100MTN1 identified 189 differentially expressed genes, with downregulation of genes involved in DNA replication, translation, and membrane transport, and upregulation of those linked to secondary metabolism and oxidative stress. KEGG pathway mapping further supported the possible causes of disruptions within the pathogen. Molecular docking suggested that the B. atrophaeus 100MTN1 derived metabolite, 6-Hydroxy-3′-methoxyflavone and exhibits binding affinity for key Fol proteins that compares favorably with the commercial fungicides. Greenhouse trials using tomato cv. Kalyan confirmed that treatment with strain 100MTN1 was associated with reduced disease severity and enhanced plant growth. These findings suggest that B. atrophaeus 100MTN1 suppresses Fol FOLViF through a combination of metabolite-driven inhibition and transcriptional interference, signifying its potential as a biological control agent for managing Fusarium wilt.

Phenotypic and genomic insights into Stenotrophomonas sepilia SMBL8: metabolic versatility, antibiotic resistance and pathogenic potential

The genome, transcriptome and metabolome of Aspergillus welwitschiae Ocstreb1 from wild halophytic rice, Oryza coarctata, indicates its distinctiveness.

An integrated omics study provides foundational insights into the endophytic potential and genomic distinctiveness of AwOcstreb1, isolated from halophytic rice, and opens new avenues for exploring A. welwitschiae for sustainable agriculture and fungal biology.

Whole-genome sequencing and characterization of Pseudomonas stutzeri P1 endophyte isolated from potato unveils plant growth-promoting and other traits

Endophytic bacteria play an important role in plant growth promotion and stress tolerance, offering sustainable alternatives to chemical inputs in agriculture. In this study, an endophytic bacterial strain P1 was isolated and identified as Pseudomonas stutzeri , a plant-associated bacterium exhibiting multiple plant growth–promoting traits (PGPTs). Biochemical (qualitative and quantitative) and in vitro analyses demonstrated nitrogen fixation, phosphate solubilization, ammonia production, indole-3-acetic acid (IAA) production, biofilm formation, and tolerance to abiotic stresses, including salinity and drought. Furthermore, the P1 strain displayed strong biocontrol activity against the fungal pathogen Fusarium oxysporum f. sp. cumini, indicating its potential to mitigate biotic stress. Whole-genome sequencing generated a high-quality complete genome of 4,758,235 bp. Functional annotation showed enrichment of metabolic pathways associated with plant-microbe interactions and environmental adaptation. Further analyses using KEGG and PGPT-pred data confirmed the presence of genes associated with direct and indirect PGPT, such as nitrogen fixation, phosphate solubilization, biofilm formation, and stress tolerance. The genome also contained genes related to CAZymes, adhesion, and motility, highlighting a strong plant association, whereas the genome lacked major virulence factors and antimicrobial traits, supporting the non-pathogenic nature of the P1 strain. Overall, these findings demonstrate the potential of P1 as a promising bioinoculant candidate for sustainable agriculture in the potato sector.

Genomic insights into the bioremediation potential of Paenibacillus taichungensis strain 53b: a multi-resistant plant growth-promoting bacterium

Characterization of a quorum-sensing communication based on gamma-butyrolactones in Rhodococcus erythropolis

This study provides novel and unexpected insights into the involvement of a LuxR homolog in regulating a QS system in Gram-positive bacteria and demonstrates that functional GBL-based QS systems are conserved and active in R. erythropolis.