Evaluation of DNA extraction from different canine lymphoma sample types for PARR analysis

Abstract

Abstract This study evaluated the performance of DNA extraction using the phenol–chloroform method across different types of canine lymphoma samples, with the aim of applying it to PARR. A total of 48 samples previously diagnosed as lymphoma were analyzed, including lymph node aspirates, cytological slides, andparaffin-embedded tissue blocks. DNA concentration was measured by spectrophotometry, and DNA suitability was confirmed by conventional PCR targeting a lymphocyte-specific endogenous control. No statistically significant differences were observed among the groups regarding DNA concentration (p > 0.05), with the following pairwise comparisons: paraffin-embedded tissue versus aspirate (p = 0.6827), aspirate versus cytological slide (p = 0.2242), and paraffin-embedded tissue versus cytological slide (p = 0.1173). All samples showed successful amplification, and the method demonstrated consistent performance, enabling the use of a single extraction protocol applicable to all three sample types, thereby showing potential as an accessible and standardizable approach for preparing samples for the PARR assay.