INTRACELLULAR CYTOKINE LEVELS AND HLA-DR EXPRESSION BY INNATE LYMPHOID CELLS IN HEALTH AND ALLERGY
TL;DR
Evaluated intracellular cytokine content of ILCs and their expression of HLA-DR in blood mononuclear cell cultures in patients with allergic diseases and in healthy controls found changes may influence the polarization of the immune response to an antigen and alter the interaction between ILCs and T cells in patients with allergic pathology compared with healthy controls.
Abstract
The growing prevalence of allergic diseases, such as bronchial asthma and atopic dermatitis, represents a pressing issue in modern medicine. In the development of allergen sensitization in these diseases, polarization of the type 2 immune response is observed in most cases; type 2 T helper cells and the recently discovered type 2 innate lymphoid cells regulate this response. The aim of the study was to evaluate the intracellular cytokine content of ILCs and their expression of HLA-DR in blood mononuclear cell cultures, with and without stimulation, in patients with allergic diseases and in healthy controls. Mononuclear cells were cultured in complete RPMI-1640 medium in the presence of LPS or PMA/ionomycin. Cell cultures without added activators served as controls. Mononuclear cell phenotyping was performed by flow cytometry. Patients with allergic diseases were found to have an increased proportion of IFNγ+ ILC1 in the absence of stimulation, as well as an increased proportion of IL-5+ ILC2 upon stimulation with PMA/ionomycin, compared with the control group. Furthermore, patients with allergic pathology showed a higher density of HLA-DR expression on ILC3 upon stimulation with LPS. Such changes may influence the polarization of the immune response to an antigen and alter the interaction between ILCs and T cells in patients with allergic pathology compared with healthy controls.