The SRY-box transcription factor 2 (SOX2)-cell division control protein 10 (CDC10) axis regulates bovine myogenesis via the Akt, ERK1/2, and p38 signaling pathway.

Abstract

Myogenesis is considered an important biological basis for meat production performance in livestock. The CDC10 gene, which is involved in cellular proliferation, is located within a genomic region associated with quantitative trait loci for growth traits in several cattle breeds. However, its potential regulatory role and underlying molecular mechanisms in bovine muscle development remain unclear. In this study, our results suggested that CDC10 may inhibit the proliferation of bovine skeletal muscle satellite cells while promoting their differentiation. We further found that the transcription factor SOX2 may contribute to the regulation of CDC10 expression through preferential binding to the motif containing the C allele of the g.61710450G > C SNP in the CDC10 promoter. Furthermore, RNA transcriptome sequencing and subsequent analyses suggested that the effects of CDC10 on bovine skeletal muscle satellite cell myogenesis may be associated with changes in the Akt, ERK1/2, and p38 signaling pathways. Collectively, these findings support a potential role for the SOX2-CDC10 regulatory axis in bovine myogenesis, possibly involving the Akt, ERK1/2, and p38 signaling pathways.