Aug 2026· Applied Biochemistry and Biotechnology· 0 citations· 19 references
Medicine
TL;DR
The Cas12a/crRNA-initiated triple self-priming cascade amplification assay for ultrasensitive and specific analysis of the mecA resistance gene exhibited high specificity, successfully discriminating MRSA from methicillin-susceptible Staphylococcus aureus and distinguishing single-base mismatches.
This work presents a broadly applicable one-pot detection strategy termed mutant scaffold-mediated RPA-CRISPR/Cas12a (MS-CRISPR), and introduces rationally designed point mutations into the crRNA scaffold to moderately attenuate Cas12a activation and cleavage kinetics.
Qing-Yang Jiang, Rui-Quan Xu, Zhi-Xin Lin et al.· Proceedings of the National...· 0 citations
A split-crRNA-based CRISPR/Cas12a assay integrated with a lateral flow assay is developed for rapid, sensitive detection of bacterial 16S rRNA within 1 h, without pre-amplification, presenting a promising approach for effective food safety monitoring and prevention of infectious diseases.
The design principles and working mechanisms of cascaded CRISPR strategies, encompassing Enzyme-Coupled cascades, Multi-effector class 2 CRISPR cascades, and Type III CRISPR-mediated cascades are introduced, including integration with digital detection platforms and AI-assistant algorithms are discussed.
Ruo-Nan He, Lu Miao, Rui-Jie Deng et al.· Biosensors & bioelectronics· 0 citations
A one-pot self-primer isothermal exponential amplification reaction (SP-EXPAR) combined with a CRISPR/Cas14a assay was developed for detecting KRAS G12C and G12D and demonstrated 100% sensitivity and 100% specificity compared with DNA sequencing.
This review synthesizes current advances aimed at enhancing the specificity of CRISPR diagnostics with particular emphasis on the pivotal role of CRISPR RNA (crRNA) engineering, and details how structural determinants of crRNA govern CRISPR-mediated target recognition and define the energetic and kinetic thresholds for...
A one-pot, contamination-free assay integrating reverse transcription loop-mediated isothermal amplification with clustered regularly interspaced short palindromic repeats (CRISPR-Cas12a) for the rapid and precise detection of the measles virus, showing strong potential for field applications in outbreak surveillance.
W. Ansari, Seung-Hyun Jung, Yeun-Jun Chung· Scientific Reports· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
