Insecticidal metabolites from Serratia marcescens associated with the fall armyworm (Spodoptera frugiperda): molecular identification, metabolomic profiling and molecular docking insights

Endophytic Paenibacillus lactis PEL6 from Mitrephora heyneana as a Source of Anti-Staphylococcus aureus Metabolites: In Vitro and In Silico Evaluation

Overall, this study identifies P. lactis PEL6 as a promising endophytic source of anti-S.

Gas chromatography–mass spectrometry analysis of the secondary metabolites produced by Bacillus paramycoides BOK01 and Microbacterium barkeri BPATH02 and investigation of their antifungal activity against tea pathogens

Endophytic fungus Muyocopron dipterocarpi isolated from Catharanthus roseus as a novel producer of ajmalicine and other metabolites: insights from HPLC and LC-HRMS analysis

Phytochemical profiling and insecticidal activity of Moricandia sinaica across growth stages: experimental and molecular docking insights.

BACKGROUND Stored food products are highly vulnerable to infestation by insects and mites, resulting in significant postharvest losses worldwide. Plant-derived bioactive compounds represent promising eco-friendly alternatives to synthetic pesticides. This study investigated the phytochemical composition of Moricandia sinaica at different growth stages and evaluated its insecticidal activity against Plodia interpunctella larvae, complemented by molecular docking analysis. RESULTS Gas chromatography-mass spectrometry analysis revealed clear stage-dependent variations in volatile composition. At the flowering stage, hexahydrofarnesylacetone, 1,8-cineole, and α-thujene were predominant, whereas mature plants were characterized mainly by hexahydrofarnesylacetone, α-pinene, and dihydroactinidiolide. Fatty acid analysis showed that palmitic acid remained the dominant component, although it decreased, while oleic and stearic acids increased at maturity. Insecticidal bioassays showed concentration- and time-related increases in larval mortality against P. interpunctella larvae. Molecular docking suggested that major fatty acids may interact with residues located within the carbohydrate-recognition region of β-1,3-glucan-binding protein 3 (GNBP3), providing a predictive hypothesis that requires further experimental validation. CONCLUSION The results highlight Moricandia sinaica as a promising source of bioactive compounds with potential applications in environmentally friendly pest management strategies for stored products. © 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Antifungal Agents of Bacillus velezensis 5RB: Complex Action of Lipopeptides, Polyketides, and Subtilosin A

Bacillus velezensis is a promising candidate for the biocontrol of plant pathogens due to its production of broad-spectrum antimicrobial metabolites. The present study evaluates the antifungal potential and metabolic profile of the non-pathogenic rhizobacterial strain Bacillus velezensis 5RB. Modifying the mineral composition across three different growth media (A, B, and C) resulted in distinct secondary-metabolite profiles of B. velezensis 5RB. Notably, the free-cell supernatant of strain 5RB cultivated in medium A and C demonstrated the most potent antifungal activity against the gray mold pathogen Botrytis cinerea. For the first time, the antimicrobial metabolites produced by B. velezensis 5RB across these three media were comprehensively characterized using ultra-performance liquid chromatography-quantitative time-of-flight mass spectrometry (UHPLC-QTOF-MS). Mass spectrometric analyses (LC-ESI-MS) identified predominantly lipopeptides from the surfactin and fengycin families, alongside three classes of polyketides: macrolactins, difficidins, and bacillaenes. Multiple lipopeptide homologues, including a novel surfactin isoform (B*), were confirmed by tandem mass spectrometry (LC-ESI-MS/MS). Additionally, this study provides the first experimental evidence for subtilosin A production by B. velezensis 5RB. The multicomponent fermentation matrix of 5RB conferred robust biocontrol protection to tomato leaves against B. cinerea and Phytophthora infestans in an in vitro disease model, underscoring its strong potential for sustainable agricultural applications.

Oosporein in Phlebia centrifuga: Prediction of Key Enzymes by a Combination of Multi-Omics and Molecular Dynamics

A spore-free, high-yield, scalable production platform for oosporein was established, highlighting the potential of rare, protected fungal species as sources for valuable enzymes and bioactive secondary metabolites for efficient microbial biomanufacturing systems.