An approach for increasing throughput and effectively improving the likelihood of sampling rare events based on forming small random pools of single-flow–sorted cells, amplifying their DNA by multiple displacement amplification and sequencing all cells in the pool, creating a "mini-metagenome" is developed.
The GenomeCompendium is released, a public database and interactive analysis tool for complete prokaryotic genomes and it is shown that complex, repeat-rich genomes are more common than previously estimated.
Tiberiu Totu, Garance Jaques, B. Heiniger et al.· bioRxiv· 0 citations
The search for novel highly effective microbial strains and the engineering of polyfunctional microbial inoculants based on them represents a key vector in the development of modern agrobiotechnologies. In this study, a comprehensive taxonomic and functional characterization of nine non-axenic strains of soil filamento...
A. Temraleeva, N. Arefieva, A. O. Kamenev et al.· Limnology and Freshwater Bio...· 0 citations
A database of giant virus metagenome-assembled genomes (GVMAGs V2), comprising 8,508 species-level representatives from 18,727 GVMAGs, a sixfold increase from the previous giant virus phylogenetic frameworks is curate, serving as a foundation for expanding giant virus diversity, uncovering virus–host interactions and e...
Yumary M. Vasquez, Tiago Nardi, G. M. Terasaki et al.· Nature Microbiology· 1 citation
Abstract This study evaluates the effectiveness of Illumina-based genome skimming for barcoding myxomycete herbarium collections ranging from 29 to 91 years in age. We successfully retrieved partial sequences of the standard marker gene (nucSSU) in all cases, as well as additional markers (mtSSU, EF1a, and COI) for cer...
D. Leontyev, M. Schnittler, O. Shchepin· IMA Fungus· 0 citations
As a versatile and broadly adaptable technology, SEnd-seq provides comprehensive insights into transcriptional regulation, RNA processing, and the coordination between RNA-based processes, thereby uncovering potential targets for antibiotic development.