Advances in bovine oocyte in vitro maturation: mechanisms and strategies

Follicle Size Regulates Cytoplasmic Maturation, Lipid Content, and Embryo Cryosurvival in Bovine Oocytes Matured In‐Vitro

In‐vitro embryo production (IVP) in cattle is limited by the suboptimal competence of oocytes matured outside the follicular environment. While maturation is often achieved, incomplete cytoplasmic maturation may impair embryo development and survival. This study evaluated the influence of follicle size on oocyte cytopl...

In Vitro Development of Primordial Follicles from Human Ovarian Tissue.

Species-specific differences in cumulus support during final oocyte maturation in mice and cattle.

Findings show that cumulus-oocyte communication is regulated in a species-specific manner during IVM and identify the bovine oocyte as a more appropriate model for translational human IVM studies that depend on intact cumulus support.

Efficacy of Human Amniotic Membrane Stem Cell-Conditioned Medium on Improving In Vitro Maturation Efficiency of Mouse Oocytes

Highlights What are the main findings? Supplementation of IVM medium with 20% hAMSC-CM significantly increased meiotic maturation rates of mouse oocytes (up to 89.4%) across all PMSG priming regimens (0, 5, 10 IU). 20% hAMSC-CM combined with 5–10 IU PMSG priming markedly enhanced cleavage and blastocyst formation rates...

N-Acetyl-L-Cysteine Promotes Porcine Oocyte In Vitro Maturation and Subsequent Embryonic Development by Regulating Oxidative Stress

Simple Summary The in vitro production of porcine embryos is important for animal breeding, reproductive research, and the preservation of valuable genetic resources. However, porcine oocytes are susceptible to damage from excessive reactive oxygen species (ROS) during in vitro maturation (IVM). This study evaluated wh...

In vitro fertilisation and vitrification disrupt embryo mitochondrial function and redox balance that persists into adulthood in mice

Background and aims Assisted reproductive technologies (ART), including in vitro fertilisation (IVF), account for over 10 million births worldwide. ART-conceived young offspring show altered cardiovascular phenotypes, including cardiac remodelling and raised blood pressure, but the mechanisms remain unclear. Mitochondr...