Aug 2026· ACS Materials Letters· Vol 8, pp. 2731-2738· 0 citations· 33 references
Abstract
We have developed a one-pot, two-stage method for the synthesis of highly monodispersed fluorescent polystyrene nanoparticles with surface carboxyl functional groups. The fluorescent nanoparticles have a tunable size range of 150−300 nm and excellent photo/chemical stability. Through conjugating antibodies to the surface carboxyl groups, various lateral flow immunoassays (LFIAs) using the fluorescent nanoparticles as the reporters have been developed, achieving a visual limit of detection (LOD) of 39 mIU mL−1 using human chorionic gonadotropin as the model sample. This LOD is 8-fold and 64-fold better as compared to LFIAs using AuNPs and free dye as reporters, respectively. The LODs of our optimized LFIAs are 3 pg mL−1 for HIV-1 p24, 4 ng mL−1 for Zika NS1, and 1 and 0.5 ng mL−1 for Staphylococcus aureus enterotoxin types A and B, respectively. These LOD values are below the clinical cutoffs and are comparable to ELISA results reported in the literature.
This thesis focuses on the development of a lateral flow assay (LFA) for the rapid and instrument-free detection of polystyrene nanoplastics (PS NPs). The sensing principle is based on gold nanoparticles (AuNPs) functionalized with 11-mercaptoundecanoic acid (MUA) and goat anti-mouse IgG (GAM), forming a pH-responsive...
Nanoplastics are an emerging class of environmental contaminants whose analysis is limited by the difficulty of resolving both particle size and polymer identity at the submicrometre scale in a single high-throughput measurement. Here, a dual-parameter flow-cytometric biosensor was developed that combined polymer-selec...
Hakan Eligul, Murat Kavruk, A. Dursun et al.· Biosensors & bioelectronics· 0 citations
A two-step, size-controlled method is described for synthesizing uniform, quasi-spherical, and monodisperse gold nanoparticles (AuNPs) with diameters ranging from 15 to 22 nm, using a combination of triethylamine (TEA) and cyclodextrins (α, β, and γCD). The first step of sonication was conducted in a modified ultrasoni...
Annisa Indah Reza, M. E. Prastya, Nagatoshi Nishiwaki et al.· ACS Omega· 0 citations
Enhancing the visual detection sensitivity of point-of-care (POC) optical sensors without resorting to complex, multistep chemical or enzymatic signal amplification remains a major challenge in sensor nanotechnology. Here, we present a multifunctional bio-nanostructured hybrid platform that integrates engineered Escher...
Po-Chih Wu, Shi-Yu Tseng, Shao-Yi Hou et al.· Italian National Conference...· 0 citations
An ultrasensitive method for rapid detection of norfloxacin (NOR) is crucial for ensuring the food safety and environmental protection. Ratiometric fluorescent sensors have attracted increasing attention as an effective detection strategy. However, there is still an urgent demand to design novel multi-color fluorescent...
Rong-Rong Yang, Ya Chen, Jing-Chao Yang et al.· Journal of Hazardous Materia...· 0 citations
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