Genome-Wide Identification and Bioinformatics Analysis of Thaumatin-like Proteins in Aegiceras corniculatum

Systematic Identification of the GH28 Gene Family in the Pathogenic Fungus Cytospora pyri and Functional Verification of the Candidate Virulence Gene VP1G_08835

Findings demonstrate that GH28 genes participate in C. pyri infection and identify VP1G_08835 as an important GH28 member required for normal growth and contributing to virulence.

Genome-Wide Identification of NLP Family Genes in Cultivated Strawberry (Fragaria × ananassa Duch.) and Analysis of Their Expression Under Heat and Botrytis cinerea Stresses

Nodule inception (NIN)-like proteins (NLPs) are plant-specific transcription factors regulating nutrient absorption, growth, and stress tolerance; however, their roles in stress responses remain largely uncharacterized. Cultivated strawberry (Fragaria × ananassa ‘Camarosa’) serves as an ideal model for dissecting the evolution and function of NLP genes. In this study, 37 FaNLP genes were identified genome-wide. Phylogenetic analysis classified them into three subfamilies, which are evenly distributed across seven chromosomes. Divergent exon–intron structures and conserved motif compositions suggest functional differentiation among FaNLPs. Quantitative real-time PCR (qRT-PCR) revealed distinct expression profiles under heat stress and Botrytis cinerea infection. Notably, FaNLPs were significantly more upregulated in the cultivar ‘Shuxing’ than in ‘Benihoppe’. Heat stress inhibited photosynthesis and altered catalase activity (CAT), superoxide dismutase activities (SOD) and peroxidase activities (POD), whereas fungal infection enhanced chitinase activity in both cultivars. Comparative genomics with Arabidopsis and rice revealed strawberry-specific evolutionary patterns of NLPs. Subcellular localization prediction indicates that FaNLP proteins primarily localize to the nucleus, implying their potential roles as transcriptional regulators. This study links FaNLP sequence characteristics with stress response phenotypes, providing a foundation for elucidating NLP-mediated regulatory networks in strawberry. Future functional assays, including overexpression and knockout analyses, will further clarify the biological roles of FaNLPs.

Genome-Wide Characterization of the Botryosphaeria dothidea GH28 Family Reveals BdGH28_3 Contributes to Virulence on Chinese Hickory

Findings identify GH28 diversification as a distinctive feature of the B. dothidea genome and establish BdGH28_3 as a virulence-associated member, providing a foundation for investigating GH28-mediated pathogenicity in woody hosts.

The genome-wide analysis of the NAC gene family in Pseudoroegneria libanotica highlights the functions of PlNAC17/20 under abiotic stress conditions

Genome-Wide Characterization and Expression Profiling of the Chitinase Gene Family in Radish (Raphanus sativus L.) Under Clubroot Stress

Structural and functional insights are provided into the RsChi family and candidate targets for breeding clubroot-resistant radish cultivars are highlighted, highlighting candidate targets for breeding clubroot-resistant radish cultivars.

Insights into the dirigent gene family in banana: genome-wide identification and role in biotic and abiotic stress tolerance

A genome-wide analysis of the DIR gene family was undertaken to characterize these genes and to gain insights into their potential involvement in stress responses, with the aim of supporting the development of improved stress-resistant banana cultivars.