This benchmark study provides recommendations for library creation of representative and minimally biased metagenome shotgun sequencing, enabling insights into functional attributes of low biomass ecosystem microbial communities.
This review elucidates the impact of LRS across isolate genomics, metagenomics, and multi-omics domains and indicates that LRS is increasingly becoming the method of choice for isolate genomics and metagenomics.
Xing Rao, Yu-He Gu, Gabriella et al.· GigaScience· 0 citations
ABSTRACT Metagenomic next-generation sequencing (mNGS) is increasingly used in infectious disease diagnostics, yet how library preparation shapes the microbial, functional, and host signals recovered from clinical samples remains poorly defined. Here, we performed a within-sample parallel comparison of three mNGS libra...
Geng-Yan Luo, Jin Zhao, Lin-Lin Xie et al.· mSystems· 0 citations
How environmental factors, including temperature, pH, and burial context, influence molecular preservation and inform substrate selection, influence molecular preservation and inform substrate selection in ancient DNA research, with an emphasis on mineralized tissues and sediments.
Julius Luvanga, Abang Akwo, K. Ketu et al.· Egyptian Journal of Forensic...· 0 citations
Next-generation sequencing (NGS) has transformed soil microbial ecology by revealing taxonomic and functional diversity that was largely inaccessible through cultivation-based approaches. However, greater sequencing resolution alone does not explain why particular microbial assemblages repeatedly emerge under specific...
L. Kucher, L. Kava, L. Cepoi et al.· Diversity· 0 citations
Plasmid construction underpins molecular biology and synthetic biology, yet validation is often limited to the inserted fragment rather than the whole plasmid, and around a third of laboratory-made plasmids carry sequence errors that can affect function. Sanger sequencing scales poorly across whole plasmids, while shor...
Rebecca Wolters, J. Debler, Gabriella Go et al.· bioRxiv· 0 citations
This work presents a novel, integrative framework that connects strategic omics and sequencing technologies to combine sophisticated metagenomics studies with customized next-generation sequencing techniques to lay the groundwork for next-generation ecological predictions and precise microbiome engineering with proper...
P. Kumari, Gaurav Sharma, M. Gupta et al.· Chemical biology letters· 0 citations
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