The findings indicate that the n-butanol fraction is a promising source of antibacterial compounds for further isolation and characterization.
Abstract
Antibiotic resistance has increased the need for new antibacterial agents from natural sources. This study evaluated the antibacterial activity of ethanolic root extract fractions of Imperata cylindrica against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Root powder was extracted with 70% ethanol and sequentially fractionated using n-hexane, ethyl acetate, n-butanol, and distilled water. Antibacterial activity was determined by the disk diffusion method at concentrations of 62.5–500 mg/mL. The most active fraction was further analyzed using thin-layer chromatography and silica gel column chromatography. The n-butanol fraction exhibited the strongest antibacterial activity, producing inhibition zones of 11.50, 9.00, and 8.17 mm against S. aureus, E. coli, and P. aeruginosa, respectively, at 500 mg/mL. Column chromatography generated 92 vials that were combined into nine subfractions. These findings indicate that the n-butanol fraction is a promising source of antibacterial compounds for further isolation and characterization.
Methanolic leaf extracts of Rumex nepalensis demonstrated markedly superior antibacterial activity against the tested multidrug-resistant strains compared with root extracts and aqueous extracts, supporting the plant’s potential as a natural source of antimicrobial agents.
Addis Mekonnen Woldehana, Tadesse Alemu Terfie, A. A. Dubale et al.· Natural Product Communicatio...· 0 citations
The increasing prevalence of antimicrobial resistance has led to a more intensive search for alternative antimicrobial agents from medicinal plants. Boswellia dalzielii (Burseraceae) is traditionally used to treat infections, with previous studies reporting antibacterial activity. However, further investigation of its...
Zainab Muhammed Umar, Aliyu Nuhu· Journal of Pharmacy & Bi...· 0 citations
The study concluded that all the partitioned fractions tested displayed activity at varying concentrations against all the oral pathogens tested with ethyl acetate fraction showing potentially interesting activity and could be useful in the management of multiple antibiotic-resistant oral pathogens.
M. Osungunna, F. Akinwumi, M. Yawson et al.· Journal of Phytomedicine and...· 0 citations
Abstract The increasing resistance of Staphylococcus aureus highlights the urgent need for new antibacterial alternatives. In this context, Streptomyces, a genus of actinobacteria widely recognized for its biosynthetic potential, represents a promising source of bioactive compounds. This study evaluated the antibacteri...
G. S. Souza, F. David, W. R. S. Gomes et al.· Brazilian Journal of Biology· 0 citations
Findings indicate that N. sativa seed extracts possess antibiofilm potential against P. aeruginosa at subinhibitory concentrations, however, as this was a purely phenotypic study, the mechanisms underlying the observed inhibition remain speculative and require direct experimental investigation.
Kehinde Sowunmi, Micheal Omeyiza Ibrahim, O. Ogundele et al.· Nepal Journal of Biotechnolo...· 0 citations
It is indicated that n-hexane flower extract possesses limited antibacterial activity against S. aureus, highlighting its potential as a source of antibacterial compounds and the need for further purification and characterization of its bioactive constituents.
Yuliatin Rahman, I. K. Suada, I. Wijaya et al.· Research Horizon· 0 citations
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