Genome-wide screening of the Keio collection identifies genetic determinants of Escherichia coli sensitivity and tolerance to benzoic acid

Genome-wide screen for genes required for smooth lipopolysaccharide production in Escherichia coli K-12

A functional wbbL gene was introduced into the E. coli K-12 KEIO single-gene deletion mutant library to restore OAg synthesis, enabling genome-wide analysis of S-LPS production by screening with colicin E2 and validated with LPS silver staining.

Transposon mutagenesis uncovers the genetic landscape of streptomycin susceptibility and implicates SbmA in aminoglycoside uptake in Escherichia coli

Identification of novel inhibitors of Mycobacterium smegmatis growth through genome-wide overexpression of Cluster P3 mycobacteriophage Xavia genes.

Functional screening is extended into a lineage of actinobacteriophages that has not previously been characterized, and several proteins with no known function are identified that warrant further mechanistic analysis.

Broad adaptations of plasmid-carrying Escherichia coli and Klebsiella pneumoniae to water as a reservoir

Findings demonstrate strain-specific adaptive responses to aquatic environments and underline the context-dependent influence of plasmids in shaping adaptation.

Development of a codon-optimised AUR1 selectable marker for genetic engineering of diverse yeast species

AbA is established as a highly effective selective agent and rational recoding of AUR1* enables the development of an efficient dominant selection marker across the yeast species evaluated in this study, expanding the genetic toolkit available for non-conventional yeasts and provides a versatile solution for strain eng...

Genomic and pan-genomic analyses of Bacillus subtilis B13 provide insights into biosynthetic potential and genetic traits associated with environmental adaptation

Insight is provided into genomic features related to the biosynthetic potential, genomic plasticity, and safety profile of B13, and suggest putative determinants of environmental adaptation, while reflecting pan-genome diversity in strain-specific traits.