Skip to content

Modifying Structure–Functionality of Common Bean ( Phaseolus vulgaris L.) Protein via Cold Atmospheric Plasma Processing and Maillard Reaction

Jul 2026 · ACS Food Science & Technology · Vol 6, pp. 2507-2524 · 0 citations · 72 references

Abstract

Structural and functional modifications of common bean (Phaseolus vulgaris L.) protein were investigated using cold atmospheric plasma (CAP) and Maillard reaction (MR) with pectin. CAP treatment (20 kV, 1–5 min) significantly enhanced protein solubility and emulsifying activity. Conversely, MR (90 °C, 0.5–3 h) reduced solubility and foaming properties but improved gelling capacity, achieving the lowest gelation concentration (LGC) at 2–3 h of heating. Conformational shifts in secondary structures (α-helix, β-turn, and β-sheet) were confirmed by FTIR and correlated with protein unfolding and structural modifications, as characterized by SDS-PAGE. Zeta potential measurements indicated that CAP improved colloidal stability, whereas extended MR heating decreased stability. These findings demonstrate that CAP is an effective nonthermal tool for enhancing protein functionality, while MR is better suited for developing stable gel-based food matrices, lowering the LGC from 8% to 5%.

View source

Similar papers

Open access Sep 2026

Heat-induced structural and functional modification of peanut protein isolate for enhancing peanut butter quality and stability

Oil separation limits peanut butter quality and shelf life. This study investigated the effect of heat-induced aggregation of peanut protein isolate (PPI) at 60–100 °C on its structural, functional properties, and evaluated its application as a stabilizer in peanut butter. Thermal treatment altered PPI conformation—mos...

Tian-Long Xiao, Shi-Shuai Cui, Yi-Cheng Zheng et al. · 0 citations
Sep 2026

Effects of Astragalus polysaccharides on the heat-induced gel properties and structural changes of myofibrillar proteins in low-salt concentration and its application in luncheon meat.

This study investigated how varying levels (0-2.5%, w/w) of Astragalus polysaccharides (AP) influenced the properties of low-salt (0.3 M NaCl) myofibrillar protein (MP) gels and their application in luncheon meat. The highest gel strength and water-holding capacity were observed at a 2% addition level, reaching 89.94 g...

Meng-Ning Zhao, Ziyuan Zhang, Ji Fang et al. · 0 citations
Aug 2026

Ultrasound-assisted extraction enhances functional properties and in vitro digestibility of Pleurotus eryngii mycelium protein: The role of structural unfolding.

To valorize Pleurotus eryngii mycelium protein (PeMP) as a sustainable protein source, this study investigated the efficacy of ultrasound-assisted extraction (0-450 W) in simultaneously enhancing the extraction yield and modulating the physicochemical properties of PeMP. Results showed that ultrasound treatment at 150 ...

Kai Zhou, Qingyue Wang, Qiaozhi Li et al. · 0 citations
Open access Sep 2026

pH-Dependent Changes in the Structural and Functional Properties of Quinoa Protein Isolates in Aqueous Dispersions

Quinoa protein isolate (QPI) is an emerging plant-based ingredient with high nutritional quality and significant potential for sustainable and clean-label food formulations. However, limited understanding of the relationships among its major protein fractions, physicochemical characteristics, and functionality restrict...

Liz Astorga-Oquendo, F. M. Harte, Osvaldo Campanella · 0 citations
Sep 2026

Enhancement of capsanthin emulsions stabilized by soy protein isolate-xanthan gum complexes on the conformational changes, gelation properties and storage stability of myofibrillar protein.

This study investigated the gel-forming capacity and storage stability of myofibrillar protein (MP) affected by capsanthin emulsions (SXCEs) stabilized by soy protein isolate (SPI) and xanthan gum (XG). SPI-XG formed a stable interfacial layer through hydrophobic interactions and hydrogen bonding, increasing emulsifyin...

Tian-Xin Wang, Bei-Bei Zheng, Ya-Feng An et al. · 0 citations

We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.