The Role of Arthrobacter pascens 13LEP5 in mitigating Drought and Cold stress in Soybean (Glycine Max (L.) Merr.)

Abstract

Drought and low temperatures are major abiotic factors affecting key physiological and biochemical processes and limiting the yields of soybean (Glycine max L. Merr.). To in-crease soybean production in Europe, different agricultural strategies are applied to re-duce abiotic stress, including biostimulants. Therefore, studies on the effectiveness of local strains isolated in Europe are becoming increasingly relevant. In this study two bacterial strains Arthrobacter pascens (AP) and Bradyrhizobium japonicum (BJ) along with plant-derived protein hydrolysate (PH) were analysed with soybean plans under abiotic stress conditions in plant growth chambers. Six treatments (control; AP; BJ; PH; BJ+AP; BJ+AP+PH) were tested to evaluate biostimulation effect before stress induction (VC stage) and to determine stress reduction effect on soybeans after plants recovery period (V3 stage). Biostimulants application has positive effect on soyabean biometric parameters in early plant development stage and post stress periods. More stable long-term effect was found on structural plant development parameters, than on pigment accumulation. The best results on plant biometric parameters were found where (AP) and (BJ+AP+PH) com-bination was inoculated. (BJ+AP+PH) combination was the only effective treatment, which showed significantly different results in pigments indices, compared to the control, after stress period. Author summary Yasha Jamil: Conceptualization, Data curation, Formal analysis, Writing– original draft, Giuseppe Colla: Formal analysis, Writing– original draft, Writing– review & editing, Justina Kaziuniene: Data curation, Formal analysis, Sarune Ramoskaite :Writing– review & editing. Monika Toleikienė: Conceptualization, Data curation, Formal analysis, Writing– original draft, Funding acquisition, Supervision, Writing– review & editing.