An Orthogonal Framework for Higher-Order Structural and In Vitro Functional Comparability of Chemically Synthesized Liraglutide Relative to an rDNA-Origin Reference Product.
Orthogonal structural and functional characterization can substantially reduce residual uncertainty for synthetic peptide generics referencing recombinant products and provides a practical framework for comparability assessment of complex peptide drugs.
Aggregation and conformational dynamics are key attributes influencing the quality of peptide and protein therapeutics, yet the oligomerization pathways that underlie these behaviors remain difficult to characterize with conventional analytical tools. Here, we establish high-pressure nuclear magnetic resonance (HP-NMR)...
Xing-Jian Xu, Pablo Trigo-Mouriño, Qi Gao et al.· Analytical Chemistry· 0 citations
BACKGROUND
Phytosterols, particularly β-sitosterol, exhibit well-recognized cholesterol-lowering activity; however, their practical application is constrained by rigid molecular structures and limited structural tunability. Rational molecular modification combined with the introduction of flexible linkers may provide a...
Ya-Qing Zhang, Shilin Hu, Huijie Cao et al.· The Journal of the Science o...· 0 citations
Low-generation PAMAM dendrimers synthesized via a divergent approach from EDA cores were characterized to assess stoichiometry-dependent structural defects and product heterogeneity. MALDI-TOF MS, RP-HPLC-UV, SEC, and 1H NMR were employed as complementary techniques to assign and compare defect-related signatures, in...
Chun T. Huang, C. Petzhold· ACS Omega· 0 citations
Understanding the molecular structure of bio-oil is essential for improving upgrading strategies, predicting physicochemical properties, and designing efficient production pathways. This work introduces an integrated experimental-analytical-modeling framework that translates multimodal characterization data into larg...
Valentina Sierra-Jimenez, Aditya Sharad Shetkar, Wen-Jun Li et al.· Energy & Fuels· 0 citations
Background: Elevated triglyceride levels constitute a major risk factor for cardiovascular disease, accounting for millions of deaths annually. Current methods for triglyceride quantification rely on enzymatic assays or complex lipid panels, which present limitations in stability, cost, and turnaround time. Peptide-bas...
James Guevara Pulido, Juliana Vargas-Paguay, James Guevara-Pulido· Revista Colombiana de Cienci...· 0 citations
Ultra-short peptide coacervates (USPCs), formed via liquid-liquid phase separation (LLPS) of peptides containing five or fewer amino acids, offer a minimal platform for microreactors and for probing structure-property relationships governing LLPS and liquid-to-solid transitions (LSTs). USPCs form uniquely hydrophobic l...
Samuel Hamroff, Lucas Caire da Silva· Small· 0 citations
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