TL;DR
This chapter presents a comprehensive workflow for isolating and analyzing pancreatic cancer-derived CTCs using scRNA-seq, and addresses critical purity and cell recovery limitations, allowing for the robust characterization of rare CTC populations.
Even with the current advances in breast cancer research, many challenges remain regarding early detection and effective treatment selection for subtypes such as triple-negative breast cancer (TNBC) and HER2-positive (HER2+). Specifically, TNC is one of the most aggressive cancer subtypes and is characterized by having...
Background/Objectives: Breast cancer (BC) is the most frequently diagnosed malignancy and a leading cause of cancer-related mortality in women worldwide. This disease is highly heterogeneous and dynamic, and chromosomal instability (CIN) plays a key role in the acquisition of these traits by generating genetic diversit...
Re-analysis of single-cell RNA-sequencing data characterizes the diverse cell subtypes within the tumor and microenvironment of TNBC, supporting a luminal progenitor origin for the cancer and providing clues as to the factors involved in progression of the disease.
These findings provide a comprehensive characterization of CTCs and CTACs in PC using a multiplex biomarker panel associated with calcium signaling and immune checkpoint pathways, highlighting the pronounced heterogeneity and potential clinical relevance of these circulating cell populations.
How spatially structured cellular ecosystems, rather than individual cell types, determine therapeutic response and resistance in lung cancer is organized around a single question - and observations that are reproducible across independent cohorts and platforms are explicitly separate from those that remain confined to...
BACKGROUND Small-cell lung cancer (SCLC) is an aggressive malignancy with a poor prognosis and marked transcriptional heterogeneity that may drive distinct therapeutic vulnerabilities. Clinical translation of molecular subtyping has been limited by restricted access to tumor biopsies, particularly at relapse. METHODS W...
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