PRISM leverages multiview image projections to promote studies of cardiac lymphatics in mouse models and surface receptor distributions within spheroid models, enabling efficient surface mapping of large, intact, and uncleared biological samples across a variety of scales.
Understanding the three-dimensional organisation of the myocardium requires imaging approaches that can resolve cellular structures across the entire heart. Existing methods are limited by trade-offs between spatial resolution, imaging depth and throughput. Here we present a high-throughput imaging pipeline for high-fi...
F. Giardini, C. Palandri, Sofia Botti et al.· Journal of Physiology· 0 citations
Fully capturing the heterogeneity of biological processes remains a central challenge, as conventional confocal microscopy typically surveys large ensembles of molecules within a small volume. The development of super-resolution imaging enables the capture of images with exceptional detail. When super-resolution is com...
Wonsang Hwang, Bing-Ying Zhao, Kai Guo et al.· Science Advances· 0 citations
OpenFLR, an open-source software framework for real-time volumetric reconstruction, three-dimensional particle tracking, and calcium image processing using FLFM is presented, and a hybrid experimental-computational PSF calibration strategy and a triangulation approach to tracking are used to extract particle positions...
Raymond Adkins, R. Hausen, Jamie Noss et al.· bioRxiv· 0 citations
A rapid, large-field bimodal imaging platform that integrates conventional brightfield microscopy with a lensless IR imaging scanner, enabling whole-slide IR image stack acquisition in minutes and suggesting that this IR-based virtual staining approach could provide a fast, chemical-free alternative for anatomic pathol...
L. Duraffourg, H. Borges, M. Fernandes et al.· Frontiers in Bioengineering...· 0 citations
Visualizing macromolecules within their native cellular context by cryo-electron tomography (cryo-ET) is fundamentally limited by the trade-off between field of view and resolution: Capturing high-resolution information about biomolecules requires high magnification, which restricts the field of view and obscures the c...
Eugene Y. D. Chua, Hamidreza Rahmani, James Zhen et al.· bioRxiv· 1 citation
Quantitative phase imaging (QPI), as a label-free microscopic imaging technique, provides quantitative optical information of cells and their subcellular structures, holding significant value in biomedical research. However, existing methods still face a notable trade-off between resolution and data efficiency. Differe...
Gui-Feng Lu, Zhuo-Shi Li, Qing-Yang Fu et al.· Global Intelligent Industry...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.