A Label-Free Fluorescence Detection Strategy for Cd2⁺ Based on an Aptamer, Exonuclease I, and SYBR Gold

Label-free and enzyme-free fluorescent biosensor for ultrasensitive detection of tobramycin via cascade signal amplification.

A novel enzyme-free and label-free fluorescent biosensor based on a cascade signal amplification strategy, integrating an entropy-driven DNA circuit (EDC) with catalytic hairpin assembly (CHA) that offers a promising platform for food safety supervision and clinical diagnostics.

Base-modified aptamer-DAPI probe for label-free detection of doxorubicin in dairy and serum samples

Doxorubicin is an important chemotherapeutic agent, making its sensitive detection essential for therapeutic monitoring and food safety assessment. Although DNA aptamers exhibit high affinity and specificity toward doxorubicin, the absence of well-defined structural motifs often hampers the development of rapid, labe...

A DNAzyme-cleavage-activated turn-on fluorescence sensing platform for sensitive miRNA detection and in situ intracellular imaging.

MicroRNAs (miRNAs) are crucial regulators in tumor development, so accurately detecting and imaging them in living cells is vital for early cancer diagnosis. However, their low levels inside cells and the complex cellular environment make traditional detection methods challenging. We developed a fluorescent sensing pla...

Gold nanoparticle-based colorimetric detection of ATP via a label-free aptamer

Adenosine triphosphate (ATP) is an important detection content in many biological reaction detection. At present, the detection methods of ATP mainly focused on electrophoresis, optical analysis, chromatography, and bioluminescence. However, these methods are not widely applied due to the high cost, complicated operati...

Label-Free Fluorescence Aptasensor Based on Aptamer and SYBR Green I for Ciprofloxacin in Actual Samples

A Simple Fluorescent Probe for Biothiol Detection and Its Application Based on a Phthalimide Derivative

A novel fluorescent probe PSH was developed for the specific detection of biothiols, using phthalimide as the fluorophore and the 2,4‐dinitrobenzenesulfonyl (DNBS) group as the recognition moiety. The DNBS group causes fluorescence quenching of PSH , while biothiols trigger a nucleophilic substitution reaction...