2026· Methods in molecular biology· Vol 3032, pp.
123-132
· 0 citations
Medicine
TL;DR
This chapter describes protocols for the preparation of key reagents, the implementation of a complete phage screening workflow, phage amplification via liquid/plate/OD260 optical density methods, and chemical coupling-based phage surface modification to achieve functionalization, along with critical notes to ensure reproducibility and reliability of the experimental process.
Bacteriophages have gained renewed attention as antibacterial agents because they inhibit bacteria through mechanisms distinct from those of antibiotics. However, the practical use of natural phages remains limited by their narrow host range and reduced activity in complex environments. Although phages are abundant in...
Bacteriophages are increasingly explored and applied to combat antibiotic-resistant bacteria, yet rapid resistance development and narrow host range can limit their broader application. Engineering phage receptor-binding proteins (RBPs) to expand phage host ranges offers a promising strategy to overcome some of these...
Bosma Jaap S, Noireaux Vincent, Bowden Steven D· ACS Synthetic Biology· 0 citations
Bacteriophages are viruses that specifically infect bacteria and have emerged as promising agents for bacterial control in human, animal, and environmental fields. On the other hand, their practical application is limited by instability outside the bacterial hosts, which compromises their efficacy. The incorporation of...
Paula Rogovski, Jéssica Sueli Dos Santos Batista, André Luis Fachini de Souza et al.· Folia Microbiologica (Prague...· 0 citations
Abstract Rapid diagnosis of infectious diseases is of paramount importance to prevent or control outbreaks and pandemics. Detection of bacteria is commonly performed using culture‐based and molecular detection methods, which cannot address the need for quick, specific and cheap diagnostics. Bacteriophage‐based assays r...
Ákos Avramucz, Joseph P. Wheatley, Sahan B. W. Liyanagedera et al.· Bioengineering & Translation...· 0 citations
A robust and scalable field‐to‐genome workflow that leverages laboratory automation to screen environmental samples against a comprehensive 188‐strain bacterial host library using a 384‐well plate format is developed.
Sean Jia Le Pang, Soon Keong Wee, Bobby Guo Wei Lee et al.· Current Protocols· 0 citations
BACKGROUND
The persistence of chlorine-tolerant Escherichia coli in wastewater treatment systems, which constitutes a major hazard to public health, highlights the need for alternative biological disinfection methods like phage-based biocontrol. However, relatively few studies have evaluated well-characterized bacterio...
A. Shehata, Marwa M. Gado, Kareem Essam et al.· Microbial Pathogenesis· 0 citations
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