Functional HCEC cell lines were successfully cultured, which possessed several important characteristics required to maintain the transparency and refractive parameters of the human cornea, including hexagonal cell morphology, higher cell adhesion and proliferation ability, HCEC pump function and positive expression of several cell adhesion markers.
Purpose
The purpose of this study was to compare whether conventional marker-based assessments adequately reflect phenotypic stability during serial passaging of two established culture protocols for primary human corneal endothelial cells (CEnCs).
Methods
Primary CEnCs were isolated from paired donor corneas from 25...
Filippo Bonelli, Tom S. Bailey, Iveta Dzivite et al.· Investigative Ophthalmology...· 0 citations
The retinal pigment epithelium (RPE) plays a central role in maintaining retinal function, and its degeneration contributes to diseases such as age-related macular degeneration. The human amniotic membrane (hAM), an ethically accessible tissue with immune privilege and epithelial organization, has been proposed as a...
Roberta Pisu, Stefania Saponara, M. N. D'Alterio et al.· Scientific Reports· 0 citations
The corneal endothelium is essential for maintaining corneal transparency and visual function through regulation of stromal hydration. However, human corneal endothelial cells have limited regenerative capacity in vivo, and their loss due to aging, trauma, surgery, or diseases such as Fuchs endothelial corneal dystroph...
Ana Kolenc, Elvira Maličev, Zala Lužnik Marzidovšek· International Journal of Mol...· 0 citations
This study investigates the isolation and expansion of limbal epithelial stem cells (LESC) and limbal mesenchymal stem cells (LMSC) from human corneal donor tissue using magnetic-activated cell sorting (MACS) with ABCG2 and CD90 microbeads, respectively. The tissue was enzymatically digested, and the resulting cell pop...
M. Mueller, N. Anischenko, Katja Lemmer et al.· Experimental Eye Research· 0 citations
Choroid plexus epithelial cells (CPEC) are implicated in cerebrospinal fluid (CSF) production and the site of the inner blood-cerebrospinal fluid barrier. CPEC are specialized ependymal cells with characteristic cell junctions, intermediate filaments, and transport protein expression, including aquaporins. We have rece...
Ronja Bihlmaier, Melanie Scharr, P. Neckel et al.· Frontiers in Cellular Neuros...· 0 citations
Corneal ulcers are common in both humans and companion animals and involve epithelial or stromal defects that may progress to sight-threatening scarring or globe perforation. Both insulin-like growth factor-1 (IGF-1) signaling pathways and MYC-mediated cellular programs promote corneal epithelial proliferation and migr...
Xiao-Chen Yuan, Caitlin Dullea, Yi-Bin Han et al.· Experimental Eye Research· 0 citations
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