A highly sensitive and specific dual-readout diagnostic platform integrating recombinase-aided amplification with the CRISPR/Cas13a system, targeting the highly conserved rsmG gene of E. lenta, serves as a robust and practical tool for rapid clinical diagnosis and point-of-care triaging of E. lenta infections.
The developed RPA-CRISPR/Cas12a assay overcomes the limitations of traditional diagnostic methods and holds great promise for on-site routine screening in poultry farms and epidemiological surveillance of MDV and REV.
Rui-Ying Han, Ning Xiao, Jia-Hua Wang et al.· Analytical Biochemistry· 0 citations
Brucellosis, a zoonotic disease caused by
Brucella
species, poses significant fhealth risks to humans and animals. Current diagnostic methods suffer from limitations such as long turnaround times, reliance on specialized equipment, or insufficient sensitivity and specificity.
A one-tube RPA-CRISPR/...
Bovine babesiosis, caused by Babesia bovis, is a tick-borne hemoparasitic disease causing substantial economic losses to cattle industries worldwide. Current diagnostic methods, particularly microscopy, lack sensitivity for subclinical infections, while molecular assays require sophisticated equipment unsuitable for fi...
Yong-Chang Li, Xiu-Juan Feng, Xian-Yue Fu et al.· Frontiers in Veterinary Scie...· 0 citations
Objective. Molecular methods have revolutionized the detection of foodborne pathogens, enabling rapid and reliable identification following a pre-enrichment step. Among the available techniques for the detection of Salmonella spp., Loop-Mediated Isothermal Amplification (LAMP) stands out for its speed, simplicity, and...
A. Mancusi· Italian Journal of Food Safe...· 0 citations
Collectively, the developed single-gene RPA-CRISPR-Cas12a lateral flow assay (LFA) represents a rapid, highly sensitive, and specific point-of-care diagnostic platform, providing visual readout by the naked eye and offering significant potential for decentralized veterinary diagnostics and field-based disease surveilla...
G. B. Manjunatha Reddy, Vignesh Manivasagam, Megha V. Naragund et al.· Journal of Virological Metho...· 0 citations
ABSTRACT In 2024, Monkeypox virus (MPXV) clade I has triggered outbreaks in several countries world‐wide. MPXV clade I demonstrates enhanced virulence and transmissibility, with a case fatality rate reaching 10%. In response, we have developed a one‐pot detection assay specifically targeting MPXV clade I, combining rec...
Bo-Yi Li, Lu-Yao Liu, Kai-Kai Jin et al.· Microbial Biotechnology· 0 citations
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