TL;DR
A sustainable biocatalytic platform for nicotinic acid production is established and a generalizable immobilization strategy for whole-cell catalysis based on rationally designed organic–inorganic hybrid shells is demonstrated.
A scalable whole-cell immobilization platform through in situ assembly of recombinant Escherichia coli expressing Sulfolobus solfataricus β-glycosidase with three covalent organic frameworks that enabled the immobilization of various prokaryotic and eukaryotic microbes, demonstrating favorable universality.
Environmental pollution, particularly water contamination, continues to be a critical global challenge, thereby necessitating the development of sustainable and high-efficiency catalytic systems for wastewater treatment. In this study, silver nanoparticles (AgNPs) were synthesized via a green chemistry approach and emb...
The biocatalytic production of nicotinic acid using nitrilases has emerged as a promising alternative to conventional chemical synthesis, offering improved selectivity and environmental sustainability. In this study, we developed a continuous-flow millireactor system employing Escherichia coli cells expressing a nitril...
Efficient conversion of agricultural waste into fermentable sugars is central to sustainable second-generation biofuel technologies. This study reports the covalent immobilization of amyloglucosidase onto a magnetic silver nanoparticle (Ag-MNP) hybrid support for the saccharification of cassava peel. The Ag-MNP hybrid...
Niacin (vitamin B3) is a high-value chemical widely used in the pharmaceutical and food industries. In this study, a recombinant Escherichia coli strain expressing nitrilase from Pseudomonas putida CGMCC3830 was evaluated for the whole-cell biotransformation of high-concentration 3-cyanopyridine to niacin. Molecular do...
Cyanophycin granule polypeptide (CGP), also known as multi-l-arginyl-poly(aspartic acid), is a biodegradable biopolymer composed primarily of aspartic acid and arginine. Due to its versatile functional properties, CGP has attracted increasing interest for potential applications in food, medicine, cosmetics, agriculture...
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