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Open access 2026

First report of an efficient in vitro regeneration protocol via callus-mediated organogenesis for Limnophila rugosa

Abstract Limnophila rugosa is a valuable medicinal plant listed in the Vietnam Red Data Book and threatened by overexploitation and habitat loss, highlighting the urgent need for effective propagation and conservation strategies. This study aimed to establish an efficient in vitro regeneration protocol via callus-media...

N. Phuoc, T. Duc, T. Thang et al. · 0 citations
Sep 2026

The influence of explant type and plant growth regulators on callus induction of Malus niedzwetzkyana

Malus niedzwetzkyana is a rare and valuable wild species characterised by a high anthocyanin content. This species is of considerable interest as a promising source of bioactive compounds. In this study, we investigated the effect of explant type on callus induction and its morphological characteristics. Stems and leav...

A. Yessimseitova, Anna Maller, D. Dyussembekova et al. · 0 citations
Open access Oct 2026

Efficient In Vitro Regeneration of Gladiolus grandiflorus L. Through Direct Organogenesis and Somatic Embryogenesis

Gladiolus grandiflorus is an economically important ornamental geophyte with a low multiplication rate and high susceptibility of corms to fungal infection under field conditions, thus limiting the availability of healthy planting material. Therefore, the development of an efficient in vitro regeneration system is e...

Afeefa Nafees, A. Mujib, Yashika Bansal et al. · 0 citations
Open access Aug 2026

Optimization of Somatic Embryogenesis from Mature Explants of Gmelina arborea Roxb.: An Efficient In Vitro Regeneration System for Forestry Applications

A reproducible protocol for somatic embryogenesis was developed from mature stem and leaf explants of Gmelina arborea Roxb., an economically important forestry species. Explants were cultured on Murashige and Skoog (MS) medium supplemented with varying concentrations (0.1–5.0 mg L⁻¹) of naphthaleneacetic acid (NAA) or...

Miller Samson S., Pritha Ghosh, Veerapagu M. et al. · 0 citations
Open access Sep 2026

Optimizing In Vitro Organogenesis in Sunflower (Helianthus annuus L.) Using Cotyledon and Root Node Explants

Sunflower (Helianthus annuus L.) is widely recognized as a recalcitrant species in tissue culture, which severely restricts its micropropagation and genetic transformation efficiency. To address this limitation, this study establishes a novel, highly efficient, and reproducible in vitro organogenesis protocol tailored...

Ferzat Turan, Aysun Örçün · 0 citations
Aug 2026

Efficient somatic embryogenesis and plant regeneration in Salvia sclarea L.: Assessment of genetic fidelity and stage-specific variation in terpene profiles

A successful method for indirect somatic embryogenesis and regeneration from leaf explants of Salvia sclarea is demonstrated and will be highly valuable for the effective use of emerging biotechnological tools.

Shafali Bhasin, Koustubh Diwakar Mashakhetri, Yadunandan Sen et al. · 0 citations

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