Sustainable Fabrication of Polymeric Electrospun Nanoscaffolds Embedded with Green Synthesized Cuo Nanoparticles from Ectocarpales Extract for Enhanced Cytotoxicity and Antioxidant Activity
TL;DR
The synergistic effects between CuO NPs and the polymeric matrix resulted in a multifunctional material with optimal physicochemical properties, biocompatibility, and radical scavenging potential, positioning it as a promising candidate for tissue engineering and wound healing applications.
Abstract
This study reports the green synthesis of copper oxide nanoparticles (CuO NPs) using ultrasonic-assisted Ectocarpales macroalgal extract and their incorporation into electrospun polyvinyl alcohol/starch nanoscaffolds for biomedical applications. The biosynthesized CuO NPs exhibited characteristic UV-Vis absorption (200 nm SPR peak), monoclinic crystallinity (XRD), and functional surface groups (FT-IR), while SEM confirmed their spherical morphology (50-200 nm) and uniform dispersion within nanofibers (70-90 nm diameter). The composite scaffolds demonstrated thermal stability up to 400°C (TGA), moderate colloidal stability (zeta potential: +14.03 mV), and enhanced bioactivity. Remarkably, nanoscaffolds showed superior antioxidant capacity (IC₅₀: 15 µg/mL vs. 45 µg/mL for extract alone) in DPPH assays and promoted 3T3-L1 cell viability (114% at 100 µg/mL). The synergistic effects between CuO NPs and the polymeric matrix resulted in a multifunctional material with optimal physicochemical properties, biocompatibility, and radical scavenging potential, positioning it as a promising candidate for tissue engineering and wound healing applications.