Geographic Variation Characteristics of Endophytic Bacterial Communities in Roots of Hippophae rhamnoides subsp. sinensis Rousi in the Arid Region of Northwest China

Abstract

As an endemic woody plant resource widely distributed in the arid regions of northwestern China, Hippophae rhamnoides subsp. sinensis Rousi possesses both ecological and medicinal value. Elucidating the effects of climate and soil conditions on the composition, structure, and function of its root-associated bacterial communities is of practical significance for the development and utilization of these indigenous plant resources in this water-limited region. In this study, root samples of H. rhamnoides were collected from 12 sampling sites in the arid region of Northwest China. High-throughput amplicon sequencing was employed to examine bacterial composition, alpha and beta diversity, molecular co-occurrence networks, and PICRUSt-based functional prediction. Mantel tests and redundancy analysis (RDA) were further applied to identify what is associated with shaping bacterial community structure. The main results are summarized as follows: (1) There were significant differences in the number of ASVs among the various sampling sites, with the P8 site having the highest number (435) and the P5 site having the lowest (156). The dominant phyla in the community were Proteobacteria, Actinobacteria, and Cyanobacteria. (2) Both α and β diversity showed significant differentiation among the 12 sampling sites. The Ace and Chao1 indices of P8 sampling sites were the highest, while P5 sampling sites were the lowest. In terms of community aggregation, P7 and P12 sampling sites showed tighter clustering, whereas P4 and P5 sampling sites showed more scattered bacterial assemblages. (3) Functional prediction suggested that metabolism, environmental information processing, and genetic information processing functional potential may all be dominant across 12 sampling sites. The abundance of environmental information predicted that the processing functional potential of the P9 sampling site was higher than that of the other 11 sampling sites, while the genetic information processing functional potential was low. (4) Cluster analysis grouped the 12 sampling sites into two groups: sampling sites P10, P11, and P12 were grouped into Group 1, while the remaining 9 sampling sites were grouped into Group 2. (5) RDA revealed that altitude was associated with shaping the root endophytic bacterial community structure of H. rhamnoides, followed by soil total nitrogen. Taken together, the H. rhamnoides populations investigated and sampled in this study were predominantly distributed in neutral-to-alkaline arid soils. The relevant environmental factors are significantly correlated with the alpha diversity patterns of root endophytic bacteria of this species, and they can modulate the community assembly processes, functional allocation characteristics, and co-occurrence network structures of these root endophytic bacteria.