The results show that in a low-powered maintenance state, limiting energy-dissipating processes, such as proton-motive force loss through flagellar rotation, supports cell survival, and suggest that growth-arrested cells live at a bioenergetic knife’s edge, where an energy-dissipating process can tip the balance betwee...
Kemal Demirer, R. A. Melnyk, Hans K. Carlson et al.· bioRxiv· 0 citations
The outermost surface layers of Gram-negative bacteria determine phage access to terminal receptors, yet their genetic basis has been mapped almost exclusively in laboratory strains that lack them. Here we apply genome-wide RB-TnSeq fitness profiling to four Escherichia coli O157:H7 strains from distinct phylogenetic c...
E. O. Rivera-Lopez, Lucas Morinière, A. Kazakov et al.· bioRxiv· 0 citations
This study shows that the conserved capsule gene CpsA is not strictly required for capsule attachment but instead works together with other LytRIZCpsAIZPsr (LCP) ligases, however, it requires the essential WalRK signaling system to maintain cell envelope integrity.
J. Zik, Zeyu Fu, M. Price et al.· bioRxiv· 0 citations
A strong negative genetic interaction is identified between the genes encoding the putative capsule ligase CpsA and the WalRK histidine kinase, a component of the WalRK two-component system regulating cell wall homeostasis, showing that the conserved capsule gene CpsA is not strictly required for capsule attachment but...
J. Zik, Zeyu Fu, M. Price et al.· mBio· 0 citations
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