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Side chain assignments are essential for in-depth characterization of proteins. We present here two refined TOCSY experiments based on the use of a variant of “perfect echo” to partially suppress the negative effects arising from homonuclear 13C couplings in the indirect dimension: The iPE-(H)CCH-TOCSY for correlating...
The design and validation of a non-proprietary 19 F NMR screening library (OpenFL600) capable of probing diverse binding pockets, as demonstrated for RNA, G protein-coupled receptors, kinases, protein-protein interactions, and proteases is presented.
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