Genome editing of the rutin-degrading enzyme gene FtRDE2 reduces seed bitterness in Tartary buckwheat.
Tartary buckwheat (F. tataricum) is a climate-resilient pseudocereal, yet its global adoption is constrained by its bitter seeds. Here, we employed the CRISPR/Cas9 system to disrupt the rutin-degrading enzyme 2 gene (FtRDE2), which catalyses the hydrolysis of rutin to quercetin, a major contributor to bitterness. Two i...